|
Journal of Histochemistry and Cytochemistry, Vol. 48, 719-724, May 2000, Copyright © 2000, The Histochemical Society, Inc.
Affinity Imaging of Red Blood Cells Using an Atomic Force Microscope
Michel Grandboisa,
Wolfgang Dettmannb,
Martin Benoitb, and
Hermann E. Gaubb
a Department of Physics and Astronomy, University of MissouriColumbia, Columbia, Missouri
b Lehrstuhl für Angewandte Physik, Universität München, München, Germany
Correspondence to:
Michel Grandbois, Dept. of Physics and Astronomy, U. of MissouriColumbia, 318 Physics Bldg., Columbia, MO 65211. E-mail: grandboism@missouri.edu
We used an atomic force microscope (AFM) to produce an image of a mixed layer of group A and O red blood cells with a contrast based only on the measured strength of a specific receptorligand pair. The image was obtained by measuring and plotting for each image pixel the adhesion force between the mixed RBC layer and the AFM tip functionalized with Helix pomatia lectin. The high specificity of that lectin for the N -acetylgalactosamine-terminated glycolipids present in the membrane of group A RBCs enabled us to discriminate between the two cell populations and to produce an image based on affinity contrast. The rupture force of the adhesion events leading to the image formation were quantitatively analyzed and compared to rupture forces measured with the same AFM tip on N-acetylgalactosamine tethered to agarose beads. The mean rupture force was found to be 65 pN when measured on the group A RBCs and 35 pN on the agarose beads. These results show that the adhesion, mediated by only a few receptorligand pairs, produces sufficient contrast for the affinity image formation. (J Histochem Cytochem 48:719724, 2000)
Key Words:
atomic force microscope (AFM), affinity, recognition, glycocalix, lectin

CiteULike Complore Connotea Del.icio.us Digg Reddit Technorati What's this?
This article has been cited by other articles:

|
 |

|
 |
 
J. Schmitz, M. Benoit, and K.-E. Gottschalk
The Viscoelasticity of Membrane Tethers and Its Importance for Cell Adhesion
Biophys. J.,
August 1, 2008;
95(3):
1448 - 1459.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. Helenius, C.-P. Heisenberg, H. E. Gaub, and D. J. Muller
Single-cell force spectroscopy
J. Cell Sci.,
June 1, 2008;
121(11):
1785 - 1791.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
L. A. Chtcheglova, J. Waschke, L. Wildling, D. Drenckhahn, and P. Hinterdorfer
Nano-Scale Dynamic Recognition Imaging on Vascular Endothelial Cells
Biophys. J.,
July 15, 2007;
93(2):
L11 - L13.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
F. Postollec, W. Norde, J. de Vries, H.J. Busscher, and H.C. van der Mei
Interactive Forces between Co-aggregating and Non-co-aggregating Oral Bacterial Pairs.
J. Dent. Res.,
March 1, 2006;
85(3):
231 - 234.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Roes, F. Mumm, U. Seydel, and T. Gutsmann
Localization of the Lipopolysaccharide-binding Protein in Phospholipid Membranes by Atomic Force Microscopy
J. Biol. Chem.,
February 3, 2006;
281(5):
2757 - 2763.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Sun, J. S. Graham, B. Hegedus, F. Marga, Y. Zhang, G. Forgacs, and M. Grandbois
Multiple Membrane Tethers Probed by Atomic Force Microscopy
Biophys. J.,
December 1, 2005;
89(6):
4320 - 4329.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Sen, S. Subramanian, and D. E. Discher
Indentation and Adhesive Probing of a Cell Membrane with AFM: Theoretical Model and Experiments
Biophys. J.,
November 1, 2005;
89(5):
3203 - 3213.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
C. M. Stroh, A. Ebner, M. Geretschlager, G. Freudenthaler, F. Kienberger, A. S. M. Kamruzzahan, S. J. Smith-Gill, H. J. Gruber, and P. Hinterdorfer
Simultaneous Topography and Recognition Imaging Using Force Microscopy
Biophys. J.,
September 1, 2004;
87(3):
1981 - 1990.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
P. Desmeules, M. Grandbois, V. A. Bondarenko, A. Yamazaki, and C. Salesse
Measurement of Membrane Binding between Recoverin, a Calcium-Myristoyl Switch Protein, and Lipid Bilayers by AFM-Based Force Spectroscopy
Biophys. J.,
June 1, 2002;
82(6):
3343 - 3350.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
P. Lundberg, I. Lundgren, H. Mukohyama, P. P. Lehenkari, M. A. Horton, and U. H. Lerner
Vasoactive Intestinal Peptide (VIP)/Pituitary Adenylate Cyclase-Activating Peptide Receptor Subtypes in Mouse Calvarial Osteoblasts: Presence of VIP-2 Receptors and Differentiation-Induced Expression of VIP-1 Receptors
Endocrinology,
January 1, 2001;
142(1):
339 - 347.
[Abstract]
[Full Text]
[PDF]
|
 |
|
The Journal of Histochemistry & Cytochemistry
is owned, published, and licensed by
The Histochemical Society © 2000
|
|
|