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Journal of Histochemistry and Cytochemistry, Vol. 48, 769-780, June 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

Presence of the Kv1.5 K+ Channel in the Sinoatrial Node

Halina Dobrzynskia, Stephen M. Rotheryb, David D.R. Marplesa, Steven R. Coppenb, Yoshiko Takagishic, Haruo Honjoc, Michael M. Tamkund, Zaineb Hendersona, Itsuo Kodamac, Nicholas J. Seversb, and Mark R. Boyetta
a School of Biomedical Sciences, University of Leeds, Leeds, United Kingdom
b Cardiac Medicine, National Heart and Lung Institute, Imperial College School of Medicine, Royal Brompton Hospital, London, United Kingdom
c Research Institute of Environmental Medicine, Nagoya University, Nagoya, Japan
d Department of Physiology, Colorado State University, Boulder, Colorado

Correspondence to: Mark R. Boyett, School of Biomedical Sciences, University of Leeds, Leeds LS2 9JT, U.K. E-mail: m.r.boyett@leeds.ac.uk

The aim of this study was to establish, using immunolabeling, whether the Kv1.5 K+ channel is present in the pacemaker of the heart, the sinoatrial (SA) node. In the atrial muscle surrounding the SA node and in the SA node itself (from guinea pig and ferret), Western blotting analysis showed a major band of the expected molecular weight, ~64 kD. Confocal microscopy and immunofluorescence labeling showed Kv1.5 labeling clustered in atrial muscle but punctate in the SA node. In atrial muscle, Kv1.5 labeling was closely associated with labeling of Cx43 (gap junction protein) and DPI/II (desmosomal protein), whereas in SA node Kv1.5 labeling was closely associated with labeling of DPI/II but not labeling of Cx43 (absent in the SA node) or Cx45 (another gap junction protein present in the SA node). Electron microscopy and immunogold labeling showed that the Kv1.5 labeling in atrial muscle is preferentially associated with desmosomes rather than gap junctions. (J Histochem Cytochem 48:769–780, 2000)

Key Words: pacemaker, ion channels, desmosomes, heart


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