Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Petersson, C.
Right arrow Articles by Magnusson, K.-E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Petersson, C.
Right arrow Articles by Magnusson, K.-E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 48, 877-884, June 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

A New Method to Visualize the Helicobacter pylori-associated Lewisb-binding Adhesin Utilizing SDS-digested Freeze-fracture Replica Labeling

Christoffer Peterssona, Bertil Larssona, Jafar Mahdavib, Thomas Borénb, and Karl-Eric Magnussona
a Department of Health and Environment, Division of Medical Microbiology, Faculty of Health Sciences, Linköping University, Linköping, Sweden
b Department of Odontology, Umeå University, Umeå, Sweden

Correspondence to: Christoffer Petersson, Div. of Medical Microbiology, Faculty of Health Sciences, Linköping University, SE-581 85 Linköping, Sweden. E-mail: chrpe@ihm.liu.se

Freeze-fracture replica labeling has become a versatile tool to visualize both membrane components and other cell structures using SDS-replica cleaning before specific immunogold labeling of proteins or lipids. We report here for the first time the adoption and optimization of the method to studies of bacterial envelopes, as applied to structural analysis of the distribution of the unique BabA-adhesin of the gastric pathogen Helicobacter pylori. BabA is important for bacterial adherence to the human epithelial cell lining of the stomach. The adhesin was found to be distributed all over the bacterial cell surfaces. Our results suggest that the SDS-replica labeling allows assessment of protein localization to distinct cell compartments and analysis of co-localization with neighboring membrane structures. (J Histochem Cytochem 48:877–883, 2000)

Key Words: freeze-fracture, Helicobacter pylori, SDS-digested freeze-fracture replica labeling, Lewisb blood group, antigen-binding adhesin, BabA, bacterial envelopes, prokaryotic cells


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 MicrobiologyHome page
S. A. Frye, R. Assalkhou, R. F. Collins, R. C. Ford, C. Petersson, J. P. Derrick, and T. Tonjum
Topology of the outer-membrane secretin PilQ from Neisseria meningitidis
Microbiology, December 1, 2006; 152(12): 3751 - 3764.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
E. J. Beswick, D. A. Bland, G. Suarez, C. A. Barrera, X. Fan, and V. E. Reyes
Helicobacter pylori Binds to CD74 on Gastric Epithelial Cells and Stimulates Interleukin-8 Production
Infect. Immun., May 1, 2005; 73(5): 2736 - 2743.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. F. Cipollo, A. M. Awad, C. E. Costello, and C. B. Hirschberg
srf-3, a Mutant of Caenorhabditis elegans, Resistant to Bacterial Infection and to Biofilm Binding, Is Deficient in Glycoconjugates
J. Biol. Chem., December 17, 2004; 279(51): 52893 - 52903.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2000