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Journal of Histochemistry and Cytochemistry, Vol. 48, 1011-1020, July 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

Unaltered Distribution of Laminins, Fibronectin, and Tenascin in Celiac Intestinal Mucosa

Matti Korhonena,b, Mia Ormioa, Robert E. Burgesonc, Ismo Virtanenb, and Erkki Savilahtia
a Hospital for Children and Adolescents, Helsinki University Central Hospital, Helsinki, Finland
b Department of Anatomy, Institute of Biomedicine, University of Helsinki, Helsinki, Finland
c Cutaneous Biology Research Center, Massachusetts Central Hospital and Harvard Medical School, Charlestown, Massachusetts

Correspondence to: Matti Korhonen, Hospital for Children and Adolescents, Helsinki University Central Hospital, FIN-00290 Helsinki, Finland. E-mail: matti.korhonen@helsinki.fi

Extensive remodeling of the extracellular matrix (ECM) occurs in inflammatory tissues. The celiac lesion in the small intestine is characterized by inflammation accompanied by profound morphological alterations. We used immunohistochemistry to determine the distribution of laminin, fibronectin, and tenascin isoforms in small intestinal biopsies of untreated patients with celiac disease. In normal mucosa, the distribution of laminin isoforms defines three epithelial basement membrane (BM) zones. We found that the organization of these zones was maintained in the celiac mucosa. Thus, components of laminin-5 ({alpha}3 and ß3) were found in the surface epithelial BM, laminin {alpha}2 chain was found selectively at crypt bottoms, and laminin {alpha}5 chain was the sole {alpha}-type chain in middle crypt BMs. Likewise, the distribution of fibronectin and tenascin resembled that of the normal gut. The organization of pericryptal fibroblasts and lamina propria smooth muscle strands, as defined by immunostaining for {alpha}-smooth muscle actin, also remained unchanged in the celiac mucosa. Unexpectedly, major ECM changes were not detected in the celiac lesion. (J Histochem Cytochem 48:1011–1020, 2000)

Key Words: basement membrane, extracellular matrix, immunohistochemistry, human


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