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Journal of Histochemistry and Cytochemistry, Vol. 48, 885-892, July 2000, Copyright © 2000, The Histochemical Society, Inc.

REVIEW

Applications of Plastic Embedding to Electron Microscopic Immunocytochemistry and In Situ Hybridization in Observations of Production and Secretion of Peptide Hormones

R. Yoshiyuki Osamuraa, Yoshiko Itoha, and Akira Matsunoa
a Department of Pathology, Tokai University School of Medicine, and Department of Neurosurgery, Teikyo University Ichihara Hospital, Kanagawa, Japan

Correspondence to: R. Yoshiyuki Osamura, Dept. of Pathology, Tokai University School of Medicine, Boseidai Isehara-city, Kanagawa, Japan 259-1193. E-mail: osamura@is.icc.u-tokai.ac.jp

Plastic embedding has been used to localize various antigens in conjunction with immunohistochemistry. Peptide hormones have been among the antigens that have been studied extensively. Recent application of water-soluble plastics such as LR White and Lowicryl has extended the ranges of detectable antigens and enabled the observation of antigen–antigen or mRNA–antigen combinations. This review article deals with technical aspects, procedures, and applications in endocrine cells. (J Histochem Cytochem 48:885–891, 2000)

Key Words: immunohistochemistry, in situ hybridization, electron microscopy, plastic embedding, peptide hormones, secretory pathway, secretory granules, messenger RNA, regulated pathway, constitutive pathway


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A. Matsuno, J. Itoh, S. Takekoshi, T. Nagashima, and R. Y. Osamura
Three-dimensional Imaging of the Intracellular Localization of Growth Hormone and Prolactin and Their mRNA Using Nanocrystal (Quantum Dot) and Confocal Laser Scanning Microscopy Techniques
J. Histochem. Cytochem., July 1, 2005; 53(7): 833 - 838.
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