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Journal of Histochemistry and Cytochemistry, Vol. 48, 985-998, July 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

Immunolocalization of Tenascin-C, {alpha}9 Integrin Subunit, and {alpha}vß6 Integrin During Wound Healing in Human Oral Mucosa

Lari Häkkinena, H. Christopher Hildebranda, Alexander Berndtb, Hartwig Kosmehlb, and Hannu Larjavaa
a University of British Columbia, Faculty of Dentistry, Department of Oral Biological and Medical Sciences, Vancouver, BC, Canada
b Friedrich Schiller University, Institute of Pathology, Jena, Germany

Correspondence to: Lari Häkkinen, U. of British Columbia, Faculty of Dentistry, Dept. of Oral Biological and Medical Sciences, 2199 Wesbrook Mall, Vancouver, BC, Canada V6T 1Z3. E-mail: lhakkine@interchange.ubc.ca

Tenascin-C (TN-C) and its isoforms are multidomain extracellular matrix (ECM) proteins that are believed to be involved in the regulation of stromal–epithelial interactions. Some of the interactions between TN-C and cells are mediated by integrins. In this study we analyzed the expression of TN-C and its large molecular weight splice isoform (TN-CL) and the putative TN-C-binding {alpha}9 and {alpha}vß6 integrins during human wound repair. In 3-day-old oral mucosal wounds, immunoreactivity for {alpha}9 integrin localized abundantly at the migrating basal wound epithelial cells. TN-C and TN-CL were localized in the matrix between and underneath {alpha}9-expressing epithelial cells. In parallel with gradual downregulation of {alpha}9 integrin immunoreactivity in 7-day and older wounds, the expression of {alpha}vß6 integrin was temporarily induced. Integrin {alpha}vß6 co-localized in the same area as TN-C and TN-CL immunoreactivity at the cell–cell contacts of the basal and suprabasal cell layers of the wound epithelium. During granulation tissue formation and reorganization from 7 to 28 days after wounding, TN-C and TN-CL were abundantly localized in the granulation tissue. The findings show that TN-CL is expressed under the migrating epithelial front and in the granulation tissue during matrix deposition in wound repair. Preferential localization of {alpha}9 integrin in migrating epithelial cells and of {alpha}vß6 integrin in epithelium after wound closure suggests different functions for these integrins in wound repair.

(J Histochem Cytochem 48:985–998, 2000)

Key Words: integrins, tenascin-C, wound repair


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