Journal of Histochemistry and Cytochemistry, Vol. 48, 1111-1120, August 2000, Copyright © 2000, The Histochemical Society, Inc.
Expression of Neuronal Nitric Oxide Synthase in Several Cell Types of the Rat Gastric Epithelium
Montserrat GarcíaVitoriaa,
Carmen GarcíaCorchóna,
José A. Rodríguezb,
Fermín GarcíaAmigotc, and
María A. Burrella
a Departments of Cytology and Histology, University of Navarra, Pamplona, Spain
b Cardiology, University of Navarra, Pamplona, Spain
c Biochemistry, University of Navarra, Pamplona, Spain
Correspondence to:
María A. Burrell, Dept. of Cytology and Histology, University of Navarra, 31080 Pamplona, Spain. E-mail: mburrell@unav.es
The aim of this study was to identify which cell types of the rat gastric epithelium express neuronal nitric oxide synthase (nNOS) because the results of the previous studies have been very divergent regarding this point. By the combination of immunohistochemical (IHC) and in situ hybridization (ISH) techniques, we detected expression of nNOS in chief and mucosecretory cells of the gastric epithelium. Moreover, some gastric endocrine cells were immunoreactive for nNOS, although they could not be distinguished in sections treated with ISH techniques. The strongest signal for all antibodies in IHC techniques was obtained when microwave (MW) heating was performed before the IHC procedure. Our results indicate that in the gastric epithelium a variety of cell types are able to produce NO. The NO produced by the different cell types (chief, mucous, and endocrine) may form a complex network of paracrine communication with an important role in gastric physiology. (J Histochem Cytochem 48:11111119, 2000)
Key Words:
nitric oxide synthase, rat, gastric epithelium, chief cells, mucous cells, endocrine cells, immunohistochemistry, in situ hybridization

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