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Journal of Histochemistry and Cytochemistry, Vol. 48, 1121-1130, August 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

Immunohistochemical Localization of Protein Gene Product 9.5, Ubiquitin, and Neuropeptide Y Immunoreactivities in Epithelial and Neuroendocrine Cells from Normal and Hyperplastic Human Prostate

Rocío Martína, Benito Fraileb, François Peinadoc, María I. Arenasb, Margarita Elicesd, Lucía Alonsof, Ricardo Paniaguab, José J. Martíne, and Luis Santamaríaf
a Department of Pathology, Clínica de La Zarzuela, Madrid, Spain
b Department of Cell Biology and Genetics, University of Alcalá de Henares, Madrid, Spain
c Department of Urology, Madrid, Spain
d Department of Pathology, Madrid, Spain
e Hospital La Princesa, Department of Anaesthesiology, Hospital Ramón y Cajal, Madrid, Spain
f Department of Morphology, School of Medicine, Autonomous University, Madrid, Spain

Correspondence to: Luis Santamaría, Dept. of Morphology, School of Medicine, Autonomous University, C/ Arzobispo Morcillo, 2, E-28029 Madrid, Spain. E-mail: luis.santamaria@uam.es

This study was designed to investigate (a) the presence of protein gene product 9.5 (PGP 9.5), ubiquitin, and neuropeptide Y (NPY) in the neuroendocrine and secretory epithelium of the human normal prostate and its secretions, and (b) the changes in immunoreactivity to these proteins in men with benign prostatic hyperplasia. Western blotting and light microscopic immunohistochemistry techniques were used and the numerical density of immunoreactive neuroendocrine cells, and the volume fractions of immunostained secretory epithelium were evaluated. Western blotting revealed the presence of the three antigens in both tissue homogenates and prostate secretion. Some neuroendocrine cells immunoreacted to PGP 9.5 and NPY in all the prostate regions of control specimens. Ubiquitin immunoreactivity was detected in the nuclei from both basal cells and secretory epithelial cells. The cytoplasm of the secretory cells and the glandular lumen also showed immunostaining for the three proteins. The numerical densities of both PGP 9.5 and NPY neuroendocrine cells were lower in hyperplasia than in controls. No differences in the volume fraction occupied by epithelial immunostaining to both proteins was found between hyperplastic and control prostates. We concluded that (a) PGP 9.5 and NPY, but not ubiquitin, are common antigens in both neuroendocrine and secretory prostate cells, (b) the three immunoreactive proteins contribute to the prostate secretions, and (c) the secretion of ubiquitin is markedly diminished in the hyperplastic epithelium.

(J Histochem Cytochem 48:1121–1130, 2000)

Key Words: prostate, PGP 9.5, neuropeptide Y, ubiquitin, hyperplasia


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