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Journal of Histochemistry and Cytochemistry, Vol. 48, 1215-1222, September 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

CFTR, MDR1, and MRP1 Immunolocalization in Normal Human Nasal Respiratory Mucosa

My-Anh Wiolanda, Jocelyne Fleury–Feitha, Pascal Corlieub, Frédéric Commoc, Guy Monceauxb, Jean Lacau-St-Guilyb, and Jean-François Bernaudina
a Service d'Histologie–Biologie Tumorale, Université Pierre et Marie Curie, Paris VI, Hôpital Tenon
b Service d'Oto-Rhino-Laryngologie, Hôpital Tenon
c Laboratoire d'Anatomie-Pathologique, Hôpital Tenon, Paris, France

Correspondence to: Jean-François Bernaudin, Service d'Histologie–Biologie Tumorale, Université Pierre et Marie Curie, Paris VI, Hôpital Tenon, 4 rue de la Chine, 75020 Paris, France. Fax:+ 33 1 56 01 78 53

CFTR (cystic fibrosis transmembrane conductance regulator), MDR1 (multidrug resistance), and MRP1 (multidrug resistance-associated protein), members of the ABC transporter superfamily, possess multiple functions, particularly Cl-, anion, and glutathione conjugate transport and cell detoxification. They are also hypothesized to have a number of complementary functions. It is generally accepted that data obtained from nasal mucosa can be extrapolated to lower airway cell physiology. The aim of the present study was to investigate by immunohistochemistry the differential localization of CFTR, MDR1, and MRP1 in the normal mucosa of 10 human nasal turbinates. In ciliated epithelial cells, CFTR was inconstantly expressed at the apical cell surface, intense membranous labeling was observed for MDR1, and intense cytoplasmic labeling was observed for MRP1. In the glands, a higher level of expression was observed on serous cells, at the apical surface (for CFTR), on lateral membranes (for MDR1), and with an intracytoplasmic distribution (for MRP1). In conclusion, CFTR, MDR1 and MRP1 are expressed in the epithelium and glands of the nasal respiratory mucosa, but with different patterns of expression. These results suggest major roles for CFTR, MDR1, and MRP1 in serous glandular cells and a protective function for MDR1 and MRP1 in respiratory ciliated cells. (J Histochem Cytochem 48:1215–1222, 2000)

Key Words: CFTR, MDR1, MRP1, nasal mucosa, respiratory epithelium, immunohistochemistry


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