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Journal of Histochemistry and Cytochemistry, Vol. 48, 1233-1242, September 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

GFAP Is Expressed as a Major Soluble Pool Associated with Glucagon Secretory Granules in A-cells of Mouse Pancreas

Eugenio Bertellia, Marì Regolia, Federica Gambellib, Monica Lucattellib, Giuseppe Lungarellab, and Arnaldo Bastianinia
a Department of Biomedical Sciences, University of Siena, Siena, Italy
b Institute of General Pathology, University of Siena, Siena, Italy

Correspondence to: Eugenio Bertelli, Dept. of Biomedical Sciences, Univ. of Siena, Via Aldo Moro, I-53100 Siena, Italy.

To elucidate the role of intermediate filament proteins in endocrine cells, we investigated the expression and subcellular distribution of GFAP in mouse islets of Langerhans. For this purpose, combined immunocytochemical and biochemical analysis with a panel of antibodies was carried out to identify GFAP-immunoreactive cells in mouse endocrine pancreas. Cell fractionation into NP-40-soluble and detergent/high salt-insoluble components was performed to assess whether GFAP was located in the cytosolic and/or cytoskeletal compartments of immunoreactive cells. Immunoelectron microscopic analysis was carried out to determine the subcellular distribution of the protein. Peripheral islet cells were stained with anti-GFAP antiserum. These cells were identified as glucagon-secreting cells by immunocytochemical staining of consecutive sections with anti-somatostatin, anti-GFAP, and anti-glucagon antisera. Western blotting analysis of both NP-40-soluble and detergent/high-salt insoluble fractions of isolated islets of Langerhans allowed detection of GFAP in both cytosolic and cytoskeletal compartments. Interestingly, however, the former location was highly predominant. In addition, immunoelectron microscopy localized GFAP associated with the periphery of secretory granules. On the basis of these results, an intriguing role for GFAP in secretory events should be strongly suspected. (J Histochem Cytochem 48:1233–1242, 2000)

Key Words: pancreas, islets of Langerhans, GFAP, intermediate filaments, secretion, glucagon, mouse


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