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Journal of Histochemistry and Cytochemistry, Vol. 48, 1243-1256, September 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

Detection of Immature Dendritic Cells in the Enamel Organ of Rat Incisors by Using Anti-cystatin C and Anti-MHC Class II Immunocytochemistry

Sumio Nishikawaa and Fumie Sasakia
a Department of Biology, Tsurumi University School of Dental Medicine, Yokohama, Japan

Correspondence to: Sumio Nishikawa, Dept. of Biology, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan. Fax: +81-45-573-9599.

Dendritic cells in the enamel organ of rat incisors were examined with immunocytochemistry using an anti-cystatin C antibody for immature dendritic cells and macrophages, OX6 for MHC Class II, ED1 for macrophages and dendritic cells, and ED2 for macrophages. Single cells positive for anti-cystatin C appeared in the enamel organ in zones at which ameloblasts secrete enamel matrix proteins. They were also present in transition and enamel maturation zones. In addition, ameloblasts, osteocytes, and osteoclasts were labeled by anti-cystatin C. ED1 and ED2 immunocytochemistry revealed that there was no macrophage population in the enamel organ of secretion, transition, or enamel maturation zone. A double labeling study showed that most anti-cystatin C-positive cells in the enamel maturation zone were also positive for OX6, whereas anti-cystatin C-positive and OX6-negative cells were prevalent in the secretion zone. The results suggest that immature dendritic cells penetrate the enamel organ of the secretion zone and begin to mature in the zones of transition and enamel maturation. (J Histochem Cytochem 48:1243–1255, 2000)

Key Words: cystatin C, MHC class II, dendritic cells, amelogenesis, rat incisor, immunocytochemistry, electron microscopy


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