Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Szalay, J.
Right arrow Articles by Vazeos, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Szalay, J.
Right arrow Articles by Vazeos, R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 49, 49-66, January 2001, Copyright © 2001, The Histochemical Society, Inc.

ARTICLE

Associations of PKC Isoforms with the Cytoskeleton of B16F10 Melanoma Cells

Jeanne Szalaya, Patrick Brunob, Ravindra Bhatic, Jennifer Adjodhaa, Deirdre Schuelera, Virginia Summervillec, and Raina Vazeosa
a Queens College, Department of Biology, Flushing, New York and the Graduate Center PhD Program in Biology at the City University of New York, New York
b New York College of Osteopathic Medicine, Old Westbury, New York
c State University of New York, Health Sciences Center at Brooklyn, Brooklyn, New York

Correspondence to: Jeanne Szalay, Dept. Biology A, Queens College, 65-30 Kissena Blvd, Flushing NY 11367. E-mail: Jeanne_Szalay@qc.edu

Although PKC plays a major role in regulating the morphology and function of the cytoskeleton, little is known about in situ associations of specific isoforms with the cytoskeleton. We demonstrate that seven PKC isoforms are expressed in B16F10 melanoma cells and show different levels of induction by serum. Using cell cytoskeleton preparations (CSKs), confocal microscopy, and immunocytochemistry, all isoforms show specific patterns of localization to focal contact-like structures ({alpha}, {delta}), very small cytoplasmic granules/vesicles (all isoforms), dense ordered arrays of small granules in the perinuclear region ({alpha}, {delta}), granules/vesicles associated with a homogeneous framework in the cytoplasm adjacent to the nucleus ({gamma}), or irregular-shaped patches of granules at or near the nuclear perimeter ({eta}, {theta}). In addition, several isoforms are present as cytoplasmic granules/ vesicles in linear or curvilinear arrays ({alpha}, {delta}, {epsilon}, {theta}). When isoform localization is examined using 3.7% formaldehyde or methanol:acetone, the patterns of localization in CSKs are often difficult or impossible to detect, and many are described here for the first time. Double-labeling experiments with CSK demonstrate that PKC actin co-localizes with punctate {alpha}-rich particles above the nucleus, granules of {epsilon} throughout the cytoplasm, and with {theta} in irregular-shaped aggregates associated with the nucleus. Vimentin co-localizes with perinuclear granules of {delta} and ß2, and {alpha}-tubulin co-localizes with {theta} in structures at or near the nuclear surface and in microtubules associated with the microtubule organizing center (MTOC). In summary, the present study demonstrates that seven PKC isoforms are endogenously expressed in B16F10 melanoma cells. These isoforms show various levels of induction by serum and specific patterns of association with various components of the detergent-resistant cell cytoskeleton. (J Histochem Cytochem 49:49–65, 2001)

Key Words: cytoskeleton, B16F10 melanoma, PKC isoform localization, confocal microscopy, immunohistochemistry


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Cancer Res.Home page
S. R. Alonso, L. Tracey, P. Ortiz, B. Perez-Gomez, J. Palacios, M. Pollan, J. Linares, S. Serrano, A. I. Saez-Castillo, L. Sanchez, et al.
A High-Throughput Study in Melanoma Identifies Epithelial-Mesenchymal Transition as a Major Determinant of Metastasis
Cancer Res., April 1, 2007; 67(7): 3450 - 3460.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T. Togo
Disruption of the plasma membrane stimulates rearrangement of microtubules and lipid traffic toward the wound site
J. Cell Sci., July 1, 2006; 119(13): 2780 - 2786.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
W. Hamabe, R. Fujita, and H. Ueda
Insulin Receptor-Protein Kinase C-{gamma} Signaling Mediates Inhibition of Hypoxia-Induced Necrosis of Cortical Neurons
J. Pharmacol. Exp. Ther., June 1, 2005; 313(3): 1027 - 1034.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Ouko, T. R. Ziegler, L. H. Gu, L. M. Eisenberg, and V. W. Yang
Wnt11 Signaling Promotes Proliferation, Transformation, and Migration of IEC6 Intestinal Epithelial Cells
J. Biol. Chem., June 18, 2004; 279(25): 26707 - 26715.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. B. Hock and M. A. Brown
Nuclear Factor of Activated T Cells 2 Transactivation in Mast Cells: A NOVEL ISOFORM-SPECIFIC TRANSACTIVATION DOMAIN CONFERS UNIQUE Fc{epsilon}RI RESPONSIVENESS
J. Biol. Chem., July 11, 2003; 278(29): 26695 - 26703.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. A. Carnevale and M. K. Cathcart
Protein Kinase C {beta} Is Required for Human Monocyte Chemotaxis to MCP-1
J. Biol. Chem., July 3, 2003; 278(28): 25317 - 25322.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Nakhost, N. Kabir, P. Forscher, and W. S. Sossin
Protein Kinase C Isoforms Are Translocated to Microtubules in Neurons
J. Biol. Chem., October 18, 2002; 277(43): 40633 - 40639.
[Abstract] [Full Text] [PDF]

Home page
 Cell Growth Differ.Home page
E. Sanz-Navares, N. Fernandez, M. G. Kazanietz, and S. A. Rotenberg
Atypical Protein Kinase C{zeta} Suppresses Migration of Mouse Melanoma Cells
Cell Growth Differ., October 1, 2001; 12(10): 517 - 524.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2001