Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lobo, M. V.T.
Right arrow Articles by Bragado, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lobo, M. V.T.
Right arrow Articles by Bragado, R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 49, 1253-1260, October 2001, Copyright © 2001, The Histochemical Society, Inc.

ARTICLE

Localization of the Lipid Receptors CD36 and CLA-1/SR-BI in the Human Gastrointestinal Tract: Towards the Identification of Receptors Mediating the Intestinal Absorption of Dietary Lipids

Maria V.T. Loboa,b, Lydia Huertac, Natividad Ruiz–Velascoc,d, Emma Teixeiroe, Paloma de la Cuevac, Angel Celdránf, Antonia Martín–Hidalgoc, Miguel A. Vegac,d, and Rafael Bragadoe
a Departamento de Investigación, Hospital Ramón y Cajal
b Departamento de Biología Celular y Genética, Universidad de Alcalá
c Servicio de Bioquímica-Investigación, Hospital Ramón y Cajal
d Consejo Superior de Investigaciones Científicas (CSIS)
e Departamento de Inmunología, Fundación Jiménez Díaz
f Servicio de Cirugía Digestiva, Fundación Jiménez Díaz, Madrid, Spain

Correspondence to: Rafael Bragado, Dept. of Immunology, Fundación Jiménez Díaz, Avda. Reyes Católicos, 2, 28040 Madrid, Spain. E-mail: rbragado@fjd.es

The scavenger receptors CLA-1/SR-BI and CD36 interact with native and modified lipoproteins and with some anionic phospholipids. In addition, CD36 binds/transports long-chain free fatty acids. Recent biochemical evidences indicates that the rabbit CLA-1/SR-BI receptor can be detected in enterocytes, and previous studies showed the presence of mRNA for both CLA-1/SR-BI and CD36 in some segments of the intestinal tract. These findings prompted us to study their respective localization and distribution from the human stomach to the colorectal segments, using immunohistochemical methods. Their expression in the colorectal carcinoma-derived cell line Caco-2 was analyzed by Northern blotting. In the human intestinal tract, CLA-1/SR-BI was found in the brush-border membrane of enterocytes from the duodenum to the rectum. However, CD36 was found only in the duodenal and jejunal epithelium, whereas enterocytes from other intestinal segments were not stained. In the duodenum and jejunum, CD36 co-localized with CLA-1/SR-BI in the apical membrane of enterocytes. The gastric epithelium was immunonegative for both glycoproteins. We also found that CLA-1/SR-BI mRNA was expressed in Caco-2 cells and that its expression levels increased concomitantly with their differentiation. In contrast, the CD36 transcript was not found in this colon cell line, in agreement with the absence of this protein in colon epithelium. The specific localization of CLA-1/SR-BI and CD36 along the human gastrointestinal tract and their ability to interact with a large variety of lipids strongly support a physiological role for them in absorption of dietary lipids.

(J Histochem Cytochem 49:1253–1260, 2001)

Key Words: intestinal lipid absorption, intestinal lipid receptors, CD36, CLA-1/SR-BI


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
V. A. Drover, D. V. Nguyen, C. C. Bastie, Y. F. Darlington, N. A. Abumrad, J. E. Pessin, E. London, D. Sahoo, and M. C. Phillips
CD36 Mediates Both Cellular Uptake of Very Long Chain Fatty Acids and Their Intestinal Absorption in Mice
J. Biol. Chem., May 9, 2008; 283(19): 13108 - 13115.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
B. Sun, E. R. M. Eckhardt, S. Shetty, D. R. van der Westhuyzen, and N. R. Webb
Quantitative analysis of SR-BI-dependent HDL retroendocytosis in hepatocytes and fibroblasts
J. Lipid Res., August 1, 2006; 47(8): 1700 - 1713.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
L. Cai, E. R. M. Eckhardt, W. Shi, Z. Zhao, M. Nasser, W. J. S. de Villiers, and D. R. van der Westhuyzen
Scavenger receptor class B type I reduces cholesterol absorption in cultured enterocyte CaCo-2 cells
J. Lipid Res., February 1, 2004; 45(2): 253 - 262.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
G H Hansen, L-L Niels-Christiansen, L Immerdal, and E M Danielsen
Scavenger receptor class B type I (SR-BI) in pig enterocytes: trafficking from the brush border to lipid droplets during fat absorption
Gut, October 1, 2003; 52(10): 1424 - 1431.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
N. Bruneau, S. Richard, F. Silvy, A. Verine, and D. Lombardo
Lectin-like Ox-LDL Receptor Is Expressed in Human INT-407 Intestinal Cells: Involvement in the Transcytosis of Pancreatic Bile Salt-dependent Lipase
Mol. Biol. Cell, July 1, 2003; 14(7): 2861 - 2875.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2001