Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Underwood, R. A.
Right arrow Articles by Olerud, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Underwood, R. A.
Right arrow Articles by Olerud, J. E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 49, 1285-1292, October 2001, Copyright © 2001, The Histochemical Society, Inc.

ARTICLE

Color Subtractive–Computer-assisted Image Analysis for Quantification of Cutaneous Nerves in a Diabetic Mouse Model

Robert A. Underwooda, Nicole S. Gibranb, Lara A. Muffleya, Marcia L. Usuia, and John E. Oleruda
a Departments of Medicine Dermatology, University of Washington, Seattle, Washington
b Surgery, University of Washington, Seattle, Washington

Correspondence to: John E. Olerud, Dept. of Medicine (Dermatology), Box 356524, Seattle, WA 98195-6524.

Immunohistochemistry (IHC) is a valuable tool for labeling structures in tissue samples. Quantification of immunolabeled structures using traditional approaches has proved to be difficult. Manual counts of IHC-stained structures are inherently biased, require multiple observers, and generate qualitative data. Stereological methods provide accurate quantification but are complex and labor-intensive when staining must be compared among large numbers of samples. In an effort to quickly, objectively, and reproducibly quantify cutaneous innervation in a large number of counterstained tissue sections, we developed a color subtractive–computer-assisted image analysis (CS–CAIA) system. To develop and test the CS–CAIA method, tissue sections of diabetic (db/db) mouse skin and their wild-type (db/-) littermates were stained by IHC for the neural marker PGP 9.5. The brown-red PGP 9.5 peroxidase stain was colorimetrically isolated through a scripted process of color background removal. The remaining stain was thresholded and binarized for computer determination of nerve profile counts (number of stained regions), area fraction (total area of nerve profiles per unit area of tissue), and area density (total number of nerve profiles per unit area of tissue). Using CS–CAIA, epidermal nerve profile counts, area fraction, and area density were significantly lower in db/db compared to db/- mice.

(J Histochem Cytochem 49:1285–1291, 2001)

Key Words: quantification, image analysis, skin, epidermis, nerves, PGP 9.5, immunohistochemistry, diabetic mouse


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 DiabetesHome page
V. Brussee, G. Guo, Y. Dong, C. Cheng, J. A. Martinez, D. Smith, G. W. Glazner, P. Fernyhough, and D. W. Zochodne
Distal Degenerative Sensory Neuropathy in a Long-Term Type 2 Diabetes Rat Model
Diabetes, June 1, 2008; 57(6): 1664 - 1673.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
Y. S. Chen, S. S.M. Chung, and S. K. Chung
Noninvasive Monitoring of Diabetes-Induced Cutaneous Nerve Fiber Loss and Hypoalgesia in thy1-YFP Transgenic Mice
Diabetes, November 1, 2005; 54(11): 3112 - 3118.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Bianchi, B. Buyukakilli, M. Brines, C. Savino, G. Cavaletti, N. Oggioni, G. Lauria, M. Borgna, R. Lombardi, B. Cimen, et al.
Erythropoietin both protects from and reverses experimental diabetic neuropathy
PNAS, January 20, 2004; 101(3): 823 - 828.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. K. Sont, W. I. de Boer, W. A. A. M. van Schadewijk, K. Grunberg, J. H. J. M. van Krieken, P. S. Hiemstra, and P. J. Sterk
Fully Automated Assessment of Inflammatory Cell Counts and Cytokine Expression in Bronchial Tissue
Am. J. Respir. Crit. Care Med., June 1, 2003; 167(11): 1496 - 1503.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
E. M. Brey, Z. Lalani, C. Johnston, M. Wong, L. V. McIntire, P. J. Duke, and C. W. Patrick Jr.
Automated Selection of DAB-labeled Tissue for Immunohistochemical Quantification
J. Histochem. Cytochem., May 1, 2003; 51(5): 575 - 584.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2001