Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ljubimov, A. V.
Right arrow Articles by Kenney, M. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ljubimov, A. V.
Right arrow Articles by Kenney, M. C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 49, 1341-1350, November 2001, Copyright © 2001, The Histochemical Society, Inc.

ARTICLE

Increased Expression of Tenascin-C-binding Epithelial Integrins in Human Bullous Keratopathy Corneas

Alexander V. Ljubimova, Mehrnoosh Saghizadeha, Robert Pytelab, Dean Sheppardb, and M. Cristina Kenneya
a Ophthalmology Research Laboratories, Burns & Allen Research Institute, Cedars–Sinai Medical Center, University of California Los Angeles School of Medicine Affiliate, Los Angeles, California
b Department of Medicine and Lung Biology Center/San Francisco General Hospital, University of California San Francisco, San Francisco, California

Correspondence to: Alexander V. Ljubimov, Ophthalmology Research Laboratories, Cedars–Sinai Medical Center, 8700 Beverly Boulevard, Davis-5069, Los Angeles, CA 90048. E-mail: ljubimov@cshs.org

We previously found an abnormal deposition of an extracellular matrix glycoprotein, tenascin-C (TN-C), in human corneas with pseudophakic/aphakic bullous keratopathy (PBK/ABK). In this work, we studied cellular TN-C receptors in normal and PBK/ABK corneas. Cryostat sections of normal and PBK/ABK corneas were stained by immuno-fluorescence for TN-C receptors: {alpha}2, {alpha}8, {alpha}9, {alpha}Vß3, ß1, and ß6 integrins, and annexin II. ß6 integrin mRNA levels were assessed by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) using ß2-microglobulin gene to normalize the samples. In PBK/ABK compared to normal corneas, relatively minor changes were observed for {alpha}2 and ß1 integrins, and for annexin II. {alpha}8, {alpha}9, and ß6 subunits of TN-C receptors, {alpha}8ß1 {alpha}9ß1, and {alpha}Vß6, respectively, were absent from normal central corneas but were found in the central epithelium of PBK/ABK corneas. ß6 integrin showed the most significant accumulation. It correlated best with the expression of TN-C rather than with the expression of other {alpha}Vß6 ligands, fibronectin, and vitronectin. RT-PCR analysis also showed elevated levels of ß6 mRNA in PBK/ABK compared to normal corneas. Therefore, accumulation of TN-C in PBK/ABK corneas was accompanied by an increased expression of its three binding integrins, especially {alpha}Vß6 in the corneal epithelium. The interaction of tenascin-C with these integrins may contribute to the fibrotic process that occurs in PBK/ABK corneas.

(J Histochem Cytochem 49:1341–1350, 2001)

Key Words: bullous keratopathy, corneal epithelium, tenascin-C, {alpha}Vß6 integrin, {alpha}8 integrin, {alpha}9 integrin, annexin II, RT-PCR, immunofluorescence


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Stem CellsHome page
A. Pajoohesh-Ganji, S. Pal-Ghosh, S. J. Simmens, and M. A. Stepp
Integrins in Slow-Cycling Corneal Epithelial Cells at the Limbus in the Mouse
Stem Cells, April 1, 2006; 24(4): 1075 - 1086.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
S. Filenius, T. Tervo, and I. Virtanen
Production of Fibronectin and Tenascin Isoforms and Their Role in the Adhesion of Human Immortalized Corneal Epithelial Cells
Invest. Ophthalmol. Vis. Sci., August 1, 2003; 44(8): 3317 - 3325.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2001

 
Purchase HCS Short Course Manual on HCS site