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Journal of Histochemistry and Cytochemistry, Vol. 49, 1379-1386, November 2001, Copyright © 2001, The Histochemical Society, Inc.

ARTICLE

Detection of the Mr 110,000 Lung Resistance-related Protein LRP/MVP with Monoclonal Antibodies

Anouk B. Schroeijersa, George L. Scheffera, Anneke W. Reursa, Adriana C.L.M. Pijnenborga, Ciro Abbondanzab, Erik A.C. Wiemerc, and Rik J. Schepera
a Department of Pathology, Academic Hospital Vrije Universiteit, Amsterdam, The Netherlands
b Instituto di Patologia Generale ed Oncologia, Seconda Università Degli Studi di Napoli, Napoli, Italy
c Institute for Hematology, Erasmus University Rotterdam, Rotterdam, The Netherlands

Correspondence to: Rik J. Scheper, Academic Hospital Vrije Universiteit, Dept. of Pathology, PO Box 7057, 1007 MB Amsterdam, The Netherlands. E-mail: rj.scheper@vumc.nl

The Mr 110,000 lung resistance-related protein (LRP), also termed the major vault protein (MVP), constitutes >70% of subcellular ribonucleoprotein particles called vaults. Overexpression of LRP/MVP and vaults has been linked directly to MDR in cancer cells. Clinically, LRP/MVP expression can be of value to predict response to chemotherapy and prognosis. Monoclonal antibodies (MAbs) against LRP/MVP have played a critical role in determining the relevance of this protein in clinical drug resistance. We compared the applicability of the previously described MAbs LRP-56, LMR-5, LRP, 1027, 1032, and newly isolated MAbs MVP-9, MVP-16, MVP-18, and MVP-37 for the immunodetection of LRP/MVP by immunoblotting analysis and by immunocyto- and histochemistry. The availability of a broader panel of reagents for the specific and sensitive immunodetection of LRP/MVP should greatly facilitate biological and clinical studies of vault-related MDR.

(J Histochem Cytochem 49:1379–1385, 2001)

Key Words: lung resistance-related protein, (LRP), major vault protein (MVP), vault, multidrug resistance (MDR), monoclonal antibody (MAb)


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