Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Petraki, C. D.
Right arrow Articles by Diamandis, E. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Petraki, C. D.
Right arrow Articles by Diamandis, E. P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 49, 1431-1442, November 2001, Copyright © 2001, The Histochemical Society, Inc.

ARTICLE

The Spectrum of Human Kallikrein 6 (Zyme/Protease M/Neurosin) Expression in Human Tissues as Assessed by Immunohistochemistry

Constantina D. Petrakia, Vassiliki N. Karavanaa, Pavlos T. Skoufogiannisa, Sheila P. Littleb, David J.C. Howarthc, George M. Yousefc, and Eleftherios P. Diamandisc
a Department of Pathology, Evangelismos Hospital, Athens, Greece
b Central Nervous System Research, Lilly Research Laboratories, Indianapolis, Indiana
c Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, and Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada

Correspondence to: Eleftherios P. Diamandis, Dept. of Pathology and Laboratory Medicine, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada. E-mail: ediamandis@mtsinai.on.ca

The KLK6 gene is a new member of the human kallikrein gene family and encodes for a secreted protease, human kallikrein 6 (hK6; also known as zyme/protease M/neurosin). No study has as yet reported detailed immunohistochemical localization of hK6 in human tissues. Our purpose was to examine the expression of hK6 in human tissues by immunohistochemistry. We have analyzed 199 paraffin blocks from archival, current, and autopsy material prepared from almost every normal human tissue. We employed an hK6-specific polyclonal rabbit antibody and avidin–biotin to localize hK6 by IHC. The staining pattern, the distribution of the immunostaining, and its intensity were studied in detail. The IHC expression of zyme was generally cytoplasmic. Various normal human tissues expressed the protein abundantly. Glandular epithelia constituted the main immunoexpression sites, with representative organs being the breast, prostate, kidney, endometrium, colon, appendix, salivary glands, bile ducts, and gallbladder. The small intestine, stomach, endocervix, Fallopian tube, epididymis, bronchus, and upper respiratory tract showed a focal expression as well. Choroid plexus epithelium, peripheral nerves, and some neuroendocrine cells (including the islets of Langerhans, cells in the anterior pituitary gland, and adrenal medulla) expressed the protein strongly and diffusely. A characteristic immunostaining was observed in the Hassall's corpuscles of the thymus, the oxyphilic cells of the thyroid and parathyroid glands, the primordial follicles of the ovary, dendritic cells mainly in the spleen, and in various cells of the placenta. (J Histochem Cytochem 49:1431–1441, 2001)

Key Words: human kallikrein 6, immunohistochemical, expression, normal human tissues, secreted proteins, serine proteases, cancer biomarkers


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Cancer Res.Home page
G. Huper and J. R. Marks
Isogenic Normal Basal and Luminal Mammary Epithelial Isolated by a Novel Method Show a Differential Response to Ionizing Radiation
Cancer Res., April 1, 2007; 67(7): 2990 - 3001.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
M. H. Slagter, L. J.G. Gooren, A. Scorilas, C. D. Petraki, and E. P. Diamandis
Effects of Long-term Androgen Administration on Breast Tissue of Female-to-Male Transsexuals
J. Histochem. Cytochem., August 1, 2006; 54(8): 905 - 910.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
M.R. Darling, L. Jackson-Boeters, T.D. Daley, and E.P. Diamandis
Human Kallikrein 6 Expression in Salivary Gland Tumors
J. Histochem. Cytochem., March 1, 2006; 54(3): 337 - 342.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
C. A. Borgono, I. P. Michael, and E. P. Diamandis
Human Tissue Kallikreins: Physiologic Roles and Applications in Cancer
Mol. Cancer Res., May 1, 2004; 2(5): 257 - 280.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
L. J. Spicer
Proteolytic Degradation of Insulin-Like Growth Factor Binding Proteins by Ovarian Follicles: A Control Mechanism for Selection of Dominant Follicles
Biol Reprod, May 1, 2004; 70(5): 1223 - 1230.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
C. A. Borgono, L. Grass, A. Soosaipillai, G. M. Yousef, C. D. Petraki, D. H. C. Howarth, S. Fracchioli, D. Katsaros, and E. P. Diamandis
Human Kallikrein 14: A New Potential Biomarker for Ovarian and Breast Cancer
Cancer Res., December 15, 2003; 63(24): 9032 - 9041.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
A. Iwata, M. Maruyama, T. Akagi, T. Hashikawa, I. Kanazawa, S. Tsuji, and N. Nukina
Alpha-synuclein degradation by serine protease neurosin: implication for pathogenesis of synucleinopathies
Hum. Mol. Genet., October 16, 2003; 12(20): 2625 - 2635.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
E. Bitoun, A. Micheloni, L. Lamant, C. Bonnart, A. Tartaglia-Polcini, C. Cobbold, T. Al Saati, F. Mariotti, J. Mazereeuw-Hautier, F. Boralevi, et al.
LEKTI proteolytic processing in human primary keratinocytes, tissue distribution and defective expression in Netherton syndrome
Hum. Mol. Genet., October 1, 2003; 12(19): 2417 - 2430.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
G. M. Yousef, M.-E. Polymeris, L. Grass, A. Soosaipillai, P.-C. Chan, A. Scorilas, C. Borgono, N. Harbeck, B. Schmalfeldt, J. Dorn, et al.
Human Kallikrein 5: A Potential Novel Serum Biomarker for Breast and Ovarian Cancer
Cancer Res., July 15, 2003; 63(14): 3958 - 3965.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
G. M. Yousef, M.-E. Polymeris, G. M. Yacoub, A. Scorilas, A. Soosaipillai, C. Popalis, S. Fracchioli, D. Katsaros, and E. P. Diamandis
Parallel Overexpression of Seven Kallikrein Genes in Ovarian Cancer
Cancer Res., May 1, 2003; 63(9): 2223 - 2227.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
C. D. Petraki, V. N. Karavana, and E. P. Diamandis
Human Kallikrein 13 Expression in Normal Tissues: An Immunohistochemical Study
J. Histochem. Cytochem., April 1, 2003; 51(4): 493 - 501.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
T. Kishi, L. Grass, A. Soosaipillai, C. Shimizu-Okabe, and E. P. Diamandis
Human Kallikrein 8: Immunoassay Development and Identification in Tissue Extracts and Biological Fluids
Clin. Chem., January 1, 2003; 49(1): 87 - 96.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
C. D. Petraki, V. N. Karavana, L.-Y. Luo, and E. P. Diamandis
Human Kallikrein 10 Expression in Normal Tissues by Immunohistochemistry
J. Histochem. Cytochem., September 1, 2002; 50(9): 1247 - 1261.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. X. Gomis-Ruth, A. Bayes, G. Sotiropoulou, G. Pampalakis, T. Tsetsenis, V. Villegas, F. X. Aviles, and M. Coll
The Structure of Human Prokallikrein 6 Reveals a Novel Activation Mechanism for the Kallikrein Family
J. Biol. Chem., July 19, 2002; 277(30): 27273 - 27281.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
R. Lin, Y. Nagai, R. Sladek, Y. Bastien, J. Ho, K. Petrecca, G. Sotiropoulou, E. P. Diamandis, T. J. Hudson, and J. H. White
Expression Profiling in Squamous Carcinoma Cells Reveals Pleiotropic Effects of Vitamin D3 Analog EB1089 Signaling on Cell Proliferation, Differentiation, and Immune System Regulation
Mol. Endocrinol., June 1, 2002; 16(6): 1243 - 1256.
[Abstract] [Full Text] [PDF]

Home page
 BrainHome page
I. A. Scarisbrick, S. I. Blaber, C. F. Lucchinetti, C. P. Genain, M. Blaber, and M. Rodriguez
Activity of a newly identified serine protease in CNS demyelination
Brain, June 1, 2002; 125(6): 1283 - 1296.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2001

 
Purchase HCS Short Course Manual on HCS site