Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by His, I.
Right arrow Articles by Driouich, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by His, I.
Right arrow Articles by Driouich, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 49, 1525-1536, December 2001, Copyright © 2001, The Histochemical Society, Inc.

ARTICLE

Microscopic Studies on Mature Flax Fibers Embedded in LR White: Immunogold Localization of Cell Wall Matrix Polysaccharides

Isabelle Hisa, Christine Andème–Onzighia, Claudine Morvana, and Azeddine Driouicha
a UMR CNRS 6037, IFRMP23, Centre Commun de Microscopie Electronique, Université de Rouen, Mont Saint Aignan, France

Correspondence to: Azeddine Driouich, UMR CNRS 6037, Centre Commun de Microscopie Electronique, IFRMP23, Université de Rouen, 76821 Mont Saint Aignan Cedex, France. E-mail: adriouic@crihan.fr

Flax fibers have been the subject of many biochemical studies, which revealed that cellulose and pectins are the major constituents of their walls. In contrast, little is known about the location of these polymers within the walls of mature fibers by microscopic methods. This has been technically hampered by the very thick secondary wall of fibers, resulting in inadequate tissue preservation unsuitable for immunogold microscopy. In this study, we adapted the basic chemical fixation, dehydration and infiltration methods to achieve a good preservation of the cell structures of mature fibers and reduced damage to antigens. We were able to apply postembedding immunocytochemical techniques to map the location of various pectic epitopes within the walls of mature fibers. Our immunolabeling data show that homogalacturonans were exclusively found in the middle lamellae and the cell junctions but were not detectable in the secondary wall. In contrast, rhamnogalacturonan I (RG I)-associated epitopes, as well as galactan and arabinan epitopes, were abundantly distributed over the secondary wall of mature fibers.

(J Histochem Cytochem 49:1525–1535, 2001)

Key Words: immunocytochemistry, flax fibers, LR White, pectins, secondary walls


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
L. McCartney, A. W. Blake, J. Flint, D. N. Bolam, A. B. Boraston, H. J. Gilbert, and J. P. Knox
Differential recognition of plant cell walls by microbial xylan-specific carbohydrate-binding modules
PNAS, March 21, 2006; 103(12): 4765 - 4770.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2001