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Journal of Histochemistry and Cytochemistry, Vol. 49, 1605-1610, December 2001, Copyright © 2001, The Histochemical Society, Inc.

BRIEF REPORT

Functional Interaction of Caveolin-1 and eNOS in Myocardial Capillary Endothelium Revealed by Immunoelectron Microscopy

Michael Reinera, Wilhelm Blocha, and Klaus Addicksa
a Department of Anatomy I, University of Cologne, Cologne, Germany

Correspondence to: Michael Reiner, Dept. of Anatomy I, University of Cologne, Joseph-Stelzmann-Str. 9, 50931 Köln/Cologne, Germany. E-mail: michael.reiner@uni-koeln.de

Immunogold labeling on samples of isolated perfused rat hearts embedded by an innovative low-temperature LR White procedure provided detailed insight into the interaction of caveolin-1 and endothelial NOS in myocardial capillary endothelium at the subcellular level. Separately, the localization of caveolin-1 and eNOS at caveolae under steady state conditions was visualized. A double-labeling experiment supported their close co-localization. Short-term bradykinin stimulation caused a detectable dissociation of eNOS from caveolin and its redistribution to different cell compartments, whereas caveolin itself remained stationary at caveolae. Morphometric analysis revealed that more than 80% of detectable eNOS was co-localized with caveolin-1 at caveolae under control conditions. After brief stimulation for 2 min with 10-7 M bradykinin, only 26% of the eNOS signals were associated with caveolin-1 and randomly distributed over the endothelial cells. After stimulation, eNOS was found at the plasmalemmal and intracellular membranes, freely in the cytoplasm, and at outer mitochondrial membranes. (J Histochem Cytochem 49:1605–1609, 2001)

Key Words: electron microscopy, caveolins, NO synthases, immunogold labeling, biological membranes, signal transduction, isolated perfused rat heart


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