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Journal of Histochemistry and Cytochemistry, Vol. 49, 341-346, March 2001, Copyright © 2001, The Histochemical Society, Inc.

ARTICLE

Synaptotagmin I Expression in Mast Cells of Normal Human Tissues, Systemic Mast Cell Disease, and a Human Mast Cell Leukemia Cell Line

Noriko Kimuraa,b,c, Shin-ichiro Shiraishia,b,c, Kazutoshi Mizunashia,b,c, Hiroshi Ohtsua,b,c, and Itaru Kimuraa,b,c
a Department of Pathology (NK,SS), Second Department of Internal Medicine (KM), First Department of Pharmacology (HO), Tohoku University
b Graduate School of Medical Science, Sendai, Japan; Department of Pathology and Laboratory Medicine, Tohoku Rosai Hospital (NK), Sendai, Japan
c Department of Neurology (IK), National Yamagata Hospital, Yamagata, Japan

Correspondence to: Noriko Kimura, Dept. of Pathology and Laboratory Medicine, Tohoku Rosai Hospital, 21-3-4 Dainohara, Aoba-ku, Sendai 981-8563, Japan. E-mail: nkimura-path@tohokuh.rofuku.go.jp

Synaptotagmin I (STG I) is a Ca2+ sensor and one of the synaptic vesicle proteins that mediate exocytosis. To determine the mechanism of release of large granules from mast cells, we studied by immunohistochemistry the presence of STG I in mast cells in normal human tissues simultaneously with the mast cell markers mast cell tryptase (tryptase) and c-kit. The tumor cells of systemic mast cell disease (SMCD) and a human mast cell leukemia cell line (HMC-1) were also examined. Human mast cells in normal tissues and the tumor cells of SMCD expressed STG I as well as mast cell tryptase (tryptase) and c-kit. STG I mRNA and its products in HMC-1 were examined by RT-PCR analysis and immunocytochemistry, respectively. STG I expression in HMC-1 cells was compared with that in cells stimulated and non-stimulated by phorbol 12-myristate 13-acetate and also with that in NB-1 and PC12 cells, known to express STG I. STG I mRNA was detected in both non-stimulated and stimulated HMC-1 cells and in NB-1 and PC12 cells. STG I immunoreactivity was weaker than NB-1 or PC12 immunoreactivity. However, it increased in the stimulated HMC-1 cells. Mast cells expressed STG I in various states. STG I may mediate exocytosis of large granules in mast cells. (J Histochem Cytochem 49:341–345, 2001)

Key Words: mast cell, synaptotagmin I, c-kit, mast cell tryptase, exocytosis, immunohistochemistry, RT-PCR, systemic mast cell disease, HMC-1, PC12


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