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Journal of Histochemistry and Cytochemistry, Vol. 49, 347-354, March 2001, Copyright © 2001, The Histochemical Society, Inc.

ARTICLE

Prenatal and Adult Growth Hormone Gene Expression in Rat Lymphoid Organs

Sophie Rechera, Mireille Raccurta, Anne Lamberta, Peter E. Lobieb, Hichem C. Mertania, and Gérard Morela
a CNRS UMR 5578, Université Claude Bernard Lyon 1, Villeurbanne, France
b Institute of Molecular and Cell Biology, Republic of Singapore

Correspondence to: Gérard Morel, CNRS UMR 5578, Bât. 404, 3ème étage, Université Claude Bernard-Lyon l, 43 Bd 11 Novembre 1918, F69622 Villeurbanne Cedex, France. E-mail: Gerard.Morel@univ-lyonl.fr

Growth hormone (GH) exerts its immune effects on mature lymphocytes through an autocrine/paracrine mechanism. We investigated the prenatal synthesis of GH mRNA in rat lymphoid organs using the sensitive in situ RT-PCR methodology. We show that GH transcripts are detectable in the thymus and liver of the 18-day fetus. At this stage, all thymocytes are immature and express the GH gene. In fetal liver, GH gene expression was localized in circulating lymphocytes and in hematopoietic cells surrounding GH mRNA-negative hepatocytes. In situ GH gene expression in fetal lymphoid organs was confirmed by in vitro RT-PCR showing that the amplified product from fetal lymphoid tissues was similar to the product obtained from the pituitary. Moreover, GH gene expression was detected in the thymus, spleen, and ileum Peyer's patches of adult rat, with a localization restricted to the lymphocytes and endothelial and smooth muscle cells of blood vessels. The autocrine/paracrine expression of the GH gene by lymphoid and hematopoietic cells during fetal growth might influence the generation of regulatory cells involved in immunity and hematopoiesis. (J Histochem Cytochem 49:347–354, 2001)

Key Words: lymphoid organs, hematopoietic cells, rat fetus, growth hormone, in situ RT-PCR


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