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Journal of Histochemistry and Cytochemistry, Vol. 49, 419-432, April 2001, Copyright © 2001, The Histochemical Society, Inc.

REVIEW

The Vesiculo–Vacuolar Organelle (VVO): A New Endothelial Cell Permeability Organelle

Ann M. Dvoraka and Dian Fenga
a The Departments of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts

Correspondence to: Ann M. Dvorak, Dept. of Pathology, East Campus, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215. Fax: 617 667 2943

A newly defined endothelial cell permeability structure, termed the vesiculo–vacuolar organelle (VVO), has been identified in the microvasculature that accompanies tumors, in venules associated with allergic inflammation, and in the endothelia of normal venules. This organelle provides the major route of extravasation of macromolecules at sites of increased vascular permeability induced by vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), serotonin, and histamine in animal models. Continuity of these large sessile structures between the vascular lumen and the extracellular space has been demonstrated in kinetic studies with ultrastructural electron-dense tracers, by direct observation of tilted electron micrographs, and by ultrathin serial sections with three-dimensional computer reconstructions. Ultrastructural enzyme-affinity cytochemical and immunocytochemical studies have identified histamine and VPF/VEGF bound to VVOs in vivo in animal models in which these mediators of permeability are released from mast cells and tumor cells, respectively. The high-affinity receptor for VPF/VEGF, VEGFR-2, was localized to VVOs and their substructural components by pre-embedding ultrastructural immunonanogold and immunoperoxidase techniques. Similar methods were used to localize caveolin and vesicle-associated membrane protein (VAMP) to VVOs and caveolae, indicating a possible commonality of formation and function of VVOs to caveolae.

(J Histochem Cytochem 49:419–431, 2001)

Key Words: vesiculo-vacuolar organelle, endothelial cell, permeability, vascular permeability factor/, vascular endothelial growth, factor, venules, tumor biology, allergic inflammation, mast cell, histamine, electron microscopy


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