Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Portela–Gomes, G. M.
Right arrow Articles by Stridsberg, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Portela–Gomes, G. M.
Right arrow Articles by Stridsberg, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 49, 483-490, April 2001, Copyright © 2001, The Histochemical Society, Inc.

ARTICLE

Selective Processing of Chromogranin A in the Different Islet Cells in Human Pancreas

Guida Maria Portela–Gomesa,c and Mats Stridsbergb
a Departments of Genetics and Pathology, Unit of Pathology, University of Lisbon, Portugal
b Medical Sciences, Clinical Chemistry, University of Lisbon, Portugal
c Uppsala University Hospital, Sweden, and the Centres of Gastroenterology and of Nutrition, University of Lisbon, Portugal

Correspondence to: Mats Stridsberg, Assoc. Prof., Dept. of Medical Sciences, Clinical Chemistry, University Hospital, S-751 85 Uppsala, Sweden. E-mail: mats.stridsberg@klinkem.uas.lul.se

We studied the immunoreactivity of 12 different region-specific antibodies to the chromogranin A (CgA) molecule in the four major neuroendocrine cell types of the human pancreas by using double immunofluorescence techniques. The antibodies raised to the N-terminal and midportions of CgA showed, on the whole, stronger immunoreactivity than did the C-terminal antibodies, with a few exceptions. Often the immunoreactivity was stronger in glucagon cells. Insulin cells expressed immunoreactivity to all region-specific antibodies, but glucagon cells were nonreactive to two antibodies. Somatostatin cells reacted only with the C-terminal antibodies (amino acid sequences CgA 411–424), while PP cells were stained with four CgA region-specific antibodies between amino acid sequences 63–195. The cause of these differences may be that the CgA molecule is cleaved, partly masked, or partly translated from CgA mRNA. Microwave treatment improved only the staining with the CgA 361-372 antibodies, which indicates that masking is not the sole or entire cause. Our findings may indicate that the CgA molecule is cleaved in different ways in the various pancreatic endocrine cell types, giving rise to a variety of biologically functional fragments. (J Histochem Cytochem 49:483–490, 2001)

Key Words: chromogranin A, chromogranin A fragments, immunocytochemistry, pancreas, human


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
M. Gronberg, A. V. Tsolakis, L. Magnusson, E. T. Janson, and J. Saras
Distribution of Obestatin and Ghrelin in Human Tissues: Immunoreactive Cells in the Gastrointestinal Tract, Pancreas, and Mammary Glands
J. Histochem. Cytochem., September 1, 2008; 56(9): 793 - 801.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
E. Bertelli and M. Bendayan
Association between Endocrine Pancreas and Ductal System. More than an Epiphenomenon of Endocrine Differentiation and Development?
J. Histochem. Cytochem., September 1, 2005; 53(9): 1071 - 1086.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
G. M. Portela-Gomes and M. Stridsberg
Chromogranin A in the Human Gastrointestinal Tract: An Immunocytochemical Study with Region-specific Antibodies
J. Histochem. Cytochem., November 1, 2002; 50(11): 1487 - 1492.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
G. M. Portela-Gomes and M. Stridsberg
Region-specific Antibodies to Chromogranin B Display Various Immunostaining Patterns in Human Endocrine Pancreas
J. Histochem. Cytochem., August 1, 2002; 50(8): 1023 - 1030.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2001