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Journal of Histochemistry and Cytochemistry, Vol. 49, 603-612, May 2001, Copyright © 2001, The Histochemical Society, Inc.

ARTICLE

Single-copy Gene Detection Using Branched DNA (bDNA) In Situ Hybridization

Audrey N. Playera, Lu-Ping Shena, Daryn Kennya, Vincent P. Antaoa, and Janice A. Kolberga
a Bayer Diagnostics, Emeryville, California

Correspondence to: Lu-Ping Shen, Bayer Diagnostics, 4560 Horton Street, Emeryville, CA 94608-2916. Fax: (510) 655-7733.

We have developed a branched DNA in situ hybridization (bDNA ISH) method for detection of human papillomavirus (HPV) DNA in whole cells. Using human cervical cancer cell lines with known copies of HPV DNA, we show that the bDNA ISH method is highly sensitive, detecting as few as one or two copies of HPV DNA per cell. By modifying sample pretreatment, viral mRNA or DNA sequences can be detected using the same set of oligonucleotide probes. In experiments performed on mixed populations of cells, the bDNA ISH method is highly specific and can distinguish cells with HPV-16 from cells with HPV-18 DNA. Furthermore, we demonstrate that the bDNA ISH method provides precise localization, yielding positive signals retained within the subcellular compartments in which the target nucleic acid sequences are localized. As an effective and convenient means for nucleic acid detection, the bDNA ISH method is applicable to the detection of cancers and infectious agents. (J Histochem Cytochem 49:603–611, 2001)

Key Words: branched DNA (bDNA) signal, amplification, in situ hybridization (ISH), cervical cancer cell lines, human papillomavirus (HPV)


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