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Journal of Histochemistry and Cytochemistry, Vol. 49, 657-662, May 2001, Copyright © 2001, The Histochemical Society, Inc.

ARTICLE

Secretion of Carbonic Anhydrase Isoenzyme VI (CA VI) from Human and Rat Lingual Serous von Ebner's Glands

Jukka Leinonena, Seppo Parkkilaa,b, Kari Kaunistoa, Petri Koivunenc, and Hannu Rajaniemia
a Department of Anatomy and Cell Biology, University of Oulu, Oulu, Finland
b Department of Clinical Chemistry, University of Oulu, University of Oulu, Oulu, Finland
c Clinic of Otorhinolaryngology, Oulu University Hospital, University of Oulu, Oulu, Finland

Correspondence to: Jukka Leinonen, Dept. of Anatomy and Cell Biology, PO Box 5000, 90014 University of Oulu, Finland. Fax: 358-8-537 5172

Salivary carbonic anhydrase VI (CA VI) appears to contribute to taste function by protecting taste receptor cells (TRCs) from apoptosis. The serous von Ebner's glands locating in the posterior tongue deliver their saliva into the bottom of the trenches surrounding the TRC-rich circumvallate and foliate papillae. Because these glands deliver their saliva directly into the immediate vicinity of TRCs, we investigated whether CA VI is secreted by the von Ebner's glands, using immunochemical techniques. The immunohistochemical results showed that CA VI is present in the serous acinar cells, ductal cells, and ductal content of von Ebner's glands and in the demilune and ductal cells plus ductal content of rat lingual mucous glands. More importantly, CA VI was also detected in taste buds and in the taste pores. Western blotting of saliva collected from the orifices of human von Ebner's glands and CAs purified from rat von Ebner's glands confirmed that CA VI is expressed in these glands and secreted to the bottom of the trenches surrounding the circumvallate and foliate papillae. These findings are consistent with the hypothesis that locally secreted CA VI is implicated in the paracrine modulation of taste function and TRC apoptosis. (J Histochem Cytochem 49:657–662, 2001)

Key Words: carbonic anhydrase, human, immunohistochemistry, rat, saliva, taste, von Ebner's glands


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