Journal of Histochemistry and Cytochemistry
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Robinson, J. M.
Right arrow Articles by Cook, P. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Robinson, J. M.
Right arrow Articles by Cook, P. R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 49, 803-808, July 2001, Copyright © 2001, The Histochemical Society, Inc.

REVIEW

Correlative Fluorescence and Electron Microscopy on Ultrathin Cryosections: Bridging the Resolution Gap

John M. Robinsona, Toshihiro Takizawab, Ana Pomboc, and Peter R. Cookc
a Department of Physiology and Cell Biology, Ohio State University, Columbus, Ohio
b Department of Anatomy, Jichi Medical School, Tochigi, Japan
c Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom

Correspondence to: John M. Robinson, Dept. of Physiology and Cell Biology, Ohio State University, 302 Hamilton Hall, 1645 Neil Ave., Columbus, OH 43210. E-mail: robinson.21@osu.edu

Microscopy has become increasingly important for analysis of cells and cell function in recent years. This is due in large part to advances in light microscopy that facilitate quantitative studies and improve imaging of living cells. Analysis of fluorescence signals has often been a key feature in these advances. Such studies involve a number of techniques, including imaging of fluorescently labeled proteins in living cells, single-cell physiological experiments using fluorescent indicator probes, and immunofluorescence localization. The importance of fluorescence microscopy notwithstanding, there are instances in which electron microscopy provides unique information about cell structure and function. Correlative microscopy in which a fluorescence signal is reconciled with a signal from the electron microscope is an additional tool that can provide powerful information for cellular analysis. Here we review two different methodologies for correlative fluorescence and electron microscopy using ultrathin cryosections and the advantages attendant on this approach. (J Histochem Cytochem 49:803–808, 2001)

Key Words: correlative microscopy, ultrathin cryosections, immunocytochemistry, fluorescence microscopy, transcription factories, electron microscopy, immunogold


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
K. Cortese, A. Diaspro, and C. Tacchetti
Advanced Correlative Light/Electron Microscopy: Current Methods and New Developments Using Tokuyasu Cryosections
J. Histochem. Cytochem., December 1, 2009; 57(12): 1103 - 1112.
[Abstract] [Full Text] [PDF]

Home page
 BioinformaticsHome page
H. Peng
Bioimage informatics: a new area of engineering biology
Bioinformatics, September 1, 2008; 24(17): 1827 - 1836.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
I. K. Kandela, R. Bleher, and R. M. Albrecht
Multiple Correlative Immunolabeling for Light and Electron Microscopy Using Fluorophores and Colloidal Metal Particles
J. Histochem. Cytochem., October 1, 2007; 55(10): 983 - 990.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
W. E. Ackerman IV, J. M. Robinson, and D. A. Kniss
Despite Transcriptional and Functional Coordination, Cyclooxygenase-2 and Microsomal Prostaglandin E Synthase-1 Largely Reside in Distinct Lipid Microdomains in WISH Epithelial Cells
J. Histochem. Cytochem., November 1, 2005; 53(11): 1391 - 1401.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
G. Dellaire, R. Nisman, C. H. Eskiw, and D. P. Bazett-Jones
In situ imaging and isolation of proteins using dsDNA oligonucleotides
Nucleic Acids Res., November 23, 2004; 32(20): e165 - e165.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
T. Takizawa and J. M. Robinson
Ultrathin Cryosections: An Important Tool for Immunofluorescence and Correlative Microscopy
J. Histochem. Cytochem., June 1, 2003; 51(6): 707 - 714.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
Y. Ren, M. J. Kruhlak, and D. P. Bazett-Jones
Same Serial Section Correlative Light and Energy-filtered Transmission Electron Microscopy
J. Histochem. Cytochem., May 1, 2003; 51(5): 605 - 612.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2001

 
MARC Travel Awards to IHC&M 2010