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Journal of Histochemistry and Cytochemistry, Vol. 49, 803-808, July 2001, Copyright © 2001, The Histochemical Society, Inc.

REVIEW

Correlative Fluorescence and Electron Microscopy on Ultrathin Cryosections: Bridging the Resolution Gap

John M. Robinsona, Toshihiro Takizawab, Ana Pomboc, and Peter R. Cookc
a Department of Physiology and Cell Biology, Ohio State University, Columbus, Ohio
b Department of Anatomy, Jichi Medical School, Tochigi, Japan
c Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom

Correspondence to: John M. Robinson, Dept. of Physiology and Cell Biology, Ohio State University, 302 Hamilton Hall, 1645 Neil Ave., Columbus, OH 43210. E-mail: robinson.21@osu.edu

Microscopy has become increasingly important for analysis of cells and cell function in recent years. This is due in large part to advances in light microscopy that facilitate quantitative studies and improve imaging of living cells. Analysis of fluorescence signals has often been a key feature in these advances. Such studies involve a number of techniques, including imaging of fluorescently labeled proteins in living cells, single-cell physiological experiments using fluorescent indicator probes, and immunofluorescence localization. The importance of fluorescence microscopy notwithstanding, there are instances in which electron microscopy provides unique information about cell structure and function. Correlative microscopy in which a fluorescence signal is reconciled with a signal from the electron microscope is an additional tool that can provide powerful information for cellular analysis. Here we review two different methodologies for correlative fluorescence and electron microscopy using ultrathin cryosections and the advantages attendant on this approach. (J Histochem Cytochem 49:803–808, 2001)

Key Words: correlative microscopy, ultrathin cryosections, immunocytochemistry, fluorescence microscopy, transcription factories, electron microscopy, immunogold


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