Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hansel, D. E.
Right arrow Articles by Eipper, B. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hansel, D. E.
Right arrow Articles by Eipper, B. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 49, 833-844, July 2001, Copyright © 2001, The Histochemical Society, Inc.

ARTICLE

Kalirin, a GDP/GTP Exchange Factor of the Dbl Family, Is Localized to Nerve, Muscle, and Endocrine Tissue During Embryonic Rat Development

Donna E. Hansela, Mercedes E. Quiñonesa, Gabriele V. Ronnetta,b, and Betty A. Eipperc
a The Johns Hopkins University School of Medicine, Departments of Neuroscience, University of Connecticut Health Center, Farmington, Connecticut
b Neurology, University of Connecticut Health Center, Farmington, Connecticut
c Baltimore, Maryland, and Department of Neuroscience, University of Connecticut Health Center, Farmington, Connecticut

Correspondence to: Betty A. Eipper, Dept. of Neuroscience, MC3401, U. of Connecticut Health Center, 263 Farmington Ave., Farmington, CT 06030-3401. E-mail: eipper@uchc.edu

Kalirin, a homologue of trio and UNC-73, has been previously demonstrated to cause cytoskeletal rearrangements, enhanced outgrowth of neuritic processes, and altered secretion. In the adult rat, kalirin is specifically localized to the central nervous system, with the main adult isoform, kalirin-7, concentrated in neuronal postsynaptic densities. In this study we examined the expression of kalirin in rat tissue from embryonic Day 10 (E10) through E18, using an antibody that detects all known kalirin isoforms. Kalirin expression in the embryo was more widespread than in the adult, with localization of kalirin protein to both neuronal and non-neuronal tissue, such as muscle, lung, intestinal epithelium, and pancreas. In neurons, kalirin was localized both in cell bodies and axon processes; in muscle tissue, kalirin was highly localized to migrating myogenic cells and at muscle attachment sites. Western blotting analysis indicated that kalirin-7, the major adult isoform, was a minor component of embryonic kalirin; the main isoform expressed in the embryo was kalirin-9. This is the first identification of kalirin expression in embryonic tissue and the first demonstration of non-neuronal expression of kalirin. (J Histochem Cytochem 49:833–844, 2001)

Key Words: GEF, P-CIP10, duo, trio, UNC-73, spectrin, PAM


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
A. W. Harrington, Q. M. Li, C. Tep, J. B. Park, Z. He, and S. O. Yoon
The Role of Kalirin9 in p75/Nogo Receptor-mediated RhoA Activation in Cerebellar Granule Neurons
J. Biol. Chem., September 5, 2008; 283(36): 24690 - 24697.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
F. Ferraro, X.-M. Ma, J. A. Sobota, B. A. Eipper, and R. E. Mains
Kalirin/Trio Rho Guanine Nucleotide Exchange Factors Regulate a Novel Step in Secretory Granule Maturation
Mol. Biol. Cell, December 1, 2007; 18(12): 4813 - 4825.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. De, G. D. Ciccotosto, R. E. Mains, and B. A. Eipper
Trafficking of a Secretory Granule Membrane Protein Is Sensitive to Copper
J. Biol. Chem., August 10, 2007; 282(32): 23362 - 23371.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Chakrabarti, R. Lin, N. I. Schiller, Y. Wang, D. Koubi, Y.-X. Fan, B. B. Rudkin, G. R. Johnson, and M. R. Schiller
Critical Role for Kalirin in Nerve Growth Factor Signaling through TrkA
Mol. Cell. Biol., June 15, 2005; 25(12): 5106 - 5118.
[Abstract] [Full Text] [PDF]

Home page
 NeuroscientistHome page
C. A. Rabiner, R. E. Mains, and B. A. Eipper
Kalirin: A Dual Rho Guanine Nucleotide Exchange Factor That Is So Much More Than the Sum of Its Many Parts
Neuroscientist, April 1, 2005; 11(2): 148 - 160.
[Abstract] [PDF]

Home page
 J. Neurosci.Home page
X.-M. Ma, J. Huang, Y. Wang, B. A. Eipper, and R. E. Mains
Kalirin, a Multifunctional Rho Guanine Nucleotide Exchange Factor, Is Necessary for Maintenance of Hippocampal Pyramidal Neuron Dendrites and Dendritic Spines
J. Neurosci., November 19, 2003; 23(33): 10593 - 10603.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
P. Penzes, R. C. Johnson, V. Kambampati, R. E. Mains, and B. A. Eipper
Distinct Roles for the Two Rho GDP/GTP Exchange Factor Domains of Kalirin in Regulation of Neurite Growth and Neuronal Morphology
J. Neurosci., November 1, 2001; 21(21): 8426 - 8434.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2001