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Journal of Histochemistry and Cytochemistry, Vol. 49, 923-924, July 2001, Copyright © 2001, The Histochemical Society, Inc.

BRIEF REPORT

Protease XXIV Increases Detection of Mucin Gene Expression during In Situ Hybridization in Archival Tissue

Jeanette M. Rheinhardta and Walter E. Finkbeinera
a Department of Pathology, University of California–Davis, Davis, California

Correspondence to: Jeanette M. Rheinhardt, Medical Pathology, UCDMC, University of California, Davis, 4645 2nd Avenue, Research III, Rm 3400A, Sacramento, CA 95817. E-mail: jmrheinhardt@ucdavis.edu

Digoxigenin-labeled riboprobes of six groups of human mucins were evaluated for sensitivity in archival tissue, using protease XXIV or proteinase K during in situ hybridization. (J Histochem Cytochem 49:923–924, 2001)

Key Words: digoxigenin, mucins, riboprobes, ISH


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