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Journal of Histochemistry and Cytochemistry, Vol. 49, 1067-1072, September 2001, Copyright © 2001, The Histochemical Society, Inc.

RAPID COMMUNICATION

EnVision+, a New Dextran Polymer-based Signal Enhancement Technique for In Situ Hybridization (ISH)

Klaus Hermann Wiedorna, Torsten Goldmannb, Christof Hennec, Heike Kühlb, and Ekkehard Vollmerb
a Institut für Pathologie, Klinikum Stuttgart, Stuttgart, Germany
b Institut für Pathologie, Forschungszentrum Borstel, Borstel, Germany
c Dako Diagnostika GmbH, Hamburg, Germany

Correspondence to: Klaus Hermann Wiedorn, Institut für Pathologie, Klinikum Stuttgart, Kriegsbergstr. 60, 70174 Stuttgart, Germany. E-mail: k.wiedorn@katharinenhospital.de

Seventy paraffin-embedded cervical biopsy specimens and condylomata were tested for the presence of human papillomavirus (HPV) by conventional in situ hybridization (ISH) and ISH with subsequent signal amplification. Signal amplification was performed either by a commercial biotinyl–tyramide-based detection system [GenPoint (GP)] or by the novel two-layer dextran polymer visualization system EnVision+ (EV), in which both EV–horseradish peroxidase (EV–HRP) and EV–alkaline phosphatase (EV–AP) were applied. We could demonstrate for the first time, that EV in combination with preceding ISH results in a considerable increase in signal intensity and sensitivity without loss of specificity compared to conventional ISH. Compared to GP, EV revealed a somewhat lower sensitivity, as measured by determination of the integrated optical density (IOD) of the positively stained cells. However, EV is easier to perform, requires a shorter assay time, and does not raise the background problems that may be encountered with biotinyl–tyramide-based amplification systems. (J Histochem Cytochem 49:1067–1071, 2001)

Key Words: EnVision+, dextran polymer conjugate, biotinyl–tyramide, GenPoint+, in situ signal amplification, ISH


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