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Journal of Histochemistry and Cytochemistry, Vol. 49, 1155-1164, September 2001, Copyright © 2001, The Histochemical Society, Inc.

ARTICLE

Placental Alkaline Phosphatase Expression at the Apical and Basal Plasma Membrane in Term Villous Trophoblasts

Karl Leitnera, Roman Szlauera, Isabella Ellingera, Adolf Ellingerb, Klaus-Peter Zimmerc, and Renate Fuchsa
a Departments of Pathophysiology, Medical School at Münster University, Münster, Germany
b Histology and Embryology II, Medical School at Münster University, Münster, Germany
c University of Vienna, Vienna, Austria, and Department of Pediatrics, Medical School at Münster University, Münster, Germany

Correspondence to: Renate Fuchs, Dept. Pathophysiology, University of Vienna, Währinger Gürtel 18-20, A-1090 Vienna, Austria. E-mail: renate.fuchs@akh-wien.ac.at

Human placental alkaline phosphatase (PLAP) was localized at the apical and basal plasma membrane of syncytiotrophoblasts and at the surface of cytotrophoblasts in term chorionic villi using immunoelectron microscopy. Similarly, apical and basolateral PLAP expression was found in polarized trophoblast-derived BeWo cells. Trophoblasts isolated from term placentas exhibited mainly vesicular PLAP immunofluorescence staining immediately after isolation. After in vitro differentiation into syncytia, PLAP plasma membrane expression was upregulated and exceeded that observed in mononuclear trophoblasts. These data call for caution in using PLAP as a morphological marker to differentiate syncytiotrophoblasts from cytotrophoblasts or as a marker enzyme for placental brush-border membranes. (J Histochem Cytochem 49:1155–1164, 2001)

Key Words: human placental syncytiotrophoblasts, placental alkaline phosphatase, BeWo cells


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