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Journal of Histochemistry and Cytochemistry, Vol. 49, 1191-1192, September 2001, Copyright © 2001, The Histochemical Society, Inc.

BRIEF REPORT

Mapping of Mitochondrial Metabolic Competence by Cytochrome Oxidase and Succinic Dehydrogenase Cytochemistry

Carlo Bertoni–Freddaria, Patrizia Fattorettia, Tiziana Casolia, Giuseppina Di Stefanoa, Moreno Solazzia, Natascia Gracciottia, and Pierluigi Pompeib
a Neurobiology of Aging Laboratory, INRCA Research Department, Ancona, Italy
b Department of Pharmacological Sciences University of Camerino, Camerino, Italy

Correspondence to: Carlo Bertoni–Freddari, Neurobiology of Aging Laboratory, INRCA Research Dept., Via Birarelli 8, Ancona AN 60121, Italy.

To map the mitochondrial capacity to provide adenosine triphosphate (ATP), the activities of cytochrome oxidase (COX) and succinic dehydrogenase (SDH) were respectively evidenced by diaminobenzidine (DAB) and copper ferrocyanide cytochemical techniques in the cerebellar cortex of adult rats. Sampling of the positive mitochondria was carried out by the disector procedure. The ratio (R) overall area of the precipitates due to COX activity within the single mitochondrion/area of the same organelle was automatically calculated to estimate enzyme activity vs mitochondrial size. The number of SDH-positive mitochondria/µm3 of tissue (numeric density, Nv) was morphometrically calculated. Cytochemistry of key enzymes of the respiratory chain enables measurement of the actual capacity of individual mitochondria to provide ATP. This quantitative estimation allows morphofunctional mapping of the mitochondrial metabolic competence in discrete tissue and/or cellular compartments. (J Histochem Cytochem 49:1191–1192, 2001)

Key Words: mitochondrial mapping, adenosine triphosphate, cytochrome oxidase, succinic dehydrogenase, cytochemistry


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