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OBSERVATIONS ON THE REDUCTION AND QUANTITATION OF NEOTETRAZOLIUM

HELEN J. BURTNER 1, R. C. BAHN 1, and J. B. LONGLEY 1

1 From the Laboratory of Pathology and Histochemistry, NIAMD. National Institutes of Health, Bethesda, Margland

1. By means of chromatography two different colored products and readily separated from reduced samples of neotetrazolium chloride.

2. Determinations have been made of the absorption spectra and specific absorption coefficients of these products.

3. It has been shown that the two products do not represent succeeding stages in the reduction of neotetrazolium, but must arise front different tetrazoles.

4. The significance of these findings with respect to the use of neotetrazolium in the quantitation of reducing activities and with respect to the histochemical usefulness of ditetrazoliums in general is discussed.

Submitted on July 30, 1956


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