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DETERMINATION OF PROTEIN AND LIPID LOST DURING OSMIC ACID FIXATION OF TISSUES AND CELLULAR PARTICULATES

R. DUNCAN DALLAM 1 and RUTH CURTISS TELFER 1

1 Department of Biochemistry, University of Louisville School of Medicine, Louisville, Kentucky

Heart and kidney fragments and liver mitochondria and microsomes were prepared for electron microscopy by standard methods. The fixative, dehydration and embedding mediums each were analyzed separately for protein and lipid removed. It was demonstrated that protein and lipid were lost from mitochondria and microsomes and that protein was lost from tissue fragments during their preparation for electron microscopic examination. The significance of these results with respect to investigations concerned with the correlation of function to morphology is discussed.

Submitted on September 28, 1956


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