Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kourí, J. B.
Right arrow Articles by Abbud–Lozoya, K. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kourí, J. B.
Right arrow Articles by Abbud–Lozoya, K. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 50, 1333-1340, October 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

Modifications of Golgi Complex in Chondrocytes from Osteoarthrotic (OA) Rat Cartilage

Juan B. Kouría,b, Lourdes Rojasb, Elizabeth Péreza, and Karin A. Abbud–Lozoyaa
a Departamento de Patología Experimental, Centro de Investigación y de Estudios Avanzados (CINVESTAV-IPN), Mexico City, Mexico
b Unidad de Microscopia Electrónica, Centro de Investigación y de Estudios Avanzados (CINVESTAV-IPN), Mexico City, Mexico

Correspondence to: Juan B. Kourí, Centro de Investigación y de Estudios Avanzados (CINVESTAV-IPN), Ave. Politécnico Nacional 2508, Col. San Pedro de Zacatenco, Apdo. Postal 14-740, México DF, CP 07300. E-mail: bkouri@enigma.red.cinvestav.mx

The status of the Golgi complex in normal vs osteoarthrotic (OA) cartilage has not yet been studied. A monoclonal antibody, MAb 58-K-9, allowed scoring of Golgi labeling intensity. In addition, ultrastructural assessment enabled us to focus on the distribution and relation between the endoplasmic reticulum (ER) and Golgi membranes. The study was performed in both normal and partially menisectomized OA-induced rat cartilage 20 and 45 days after surgery. Comparing Golgi immunolabeling intensities (mean ± SEM) revealed a highly significant difference between normal (9.98 ± 1.25), 20-day (2.49 ± 0.34), and 45-day (0.82 ± 0.22) cartilage. Moreover, chondrocytes from normal cartilage displayed 71.18% of labeling intensity in contrast to OA cartilage, in which chondrocyte labeling intensities were 24.95% (20 days) and 8.11% (45 days). OA chondrocytes appeared to display an overall reduction in Golgi labeling intensity, suggesting disruption of this organelle as the OA damage progressed. Interestingly, many 20-day OA-induced chondrocytes exhibited bubble-like Golgi immunolabeling compartmentalizing the cytoplasm, concomitant with putative apoptotic nuclear changes. At the same time, OA chondrocytes with a typical ultrastructural apoptotic pattern revealed a prominent ER gathered together with Golgi vesicles and saccules, also appearing to compartmentalize chondrocyte cytoplasm. We speculate about the role of Golgi modifications and apoptosis in OA pathogenesis.

(J Histochem Cytochem 50:1333–1339, 2002)

Key Words: osteoarthrosis, Golgi, cartilage, chondrocytes, immunohistochemistry, ultrastructure


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2002

 
Purchase HCS Short Course Manual on HCS site