Journal of Histochemistry and Cytochemistry
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sompuram, S. R.
Right arrow Articles by Bogen, S. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sompuram, S. R.
Right arrow Articles by Bogen, S. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 50, 1425-1434, November 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

A Novel Quality Control Slide for Quantitative Immunohistochemistry Testing

Seshi R. Sompurama, Vani Kodelaa, Keming Zhanga, Halasya Ramanathana, Gail Radcliffea, Peter Falba, and Steven A. Bogena
a CytoLogix Corporation, Cambridge, Massachusetts

Correspondence to: Seshi R. Sompuram, CytoLogix Corp., 99 Erie St., Cambridge, MA 02139. E-mail: ssompuram@cytologix.com

We introduce a novel quality control technology that may improve intra- and interlaboratory immunohistochemistry (IHC) standardization. The technology involves the creation of standardized antibody targets that are attached to the same slides as the patient sample. After IHC staining, the targets turn the same color as the stained cells or tissue elements. Unlike current clinical practice, our proposed targets are neither cells nor tissue sections. To create reproducible standards that are available in unlimited supply, we use short constrained peptides as antibody targets. These peptides are attached directly to the glass slide. We show that these peptides simulate the portion of the native antigen to which the antibody binds. They are useful in detecting subtle changes in IHC staining efficacy. Moreover, the peptides do not degrade after deparaffinization or antigen retrieval treatments. This technology may be valuable in creating nationally standardized controls to quantify IHC analytical variability. (J Histochem Cytochem 50:1425–1433, 2002)

Key Words: quality control, immunohistochemistry, peptides


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
Y. Lu, J. Nie, X. Liu, Y. Zheng, and S.-W. Guo
Trichostatin A, a histone deacetylase inhibitor, reduces lesion growth and hyperalgesia in experimentally induced endometriosis in mice
Hum. Reprod., January 29, 2010; (2010): dep472v1 - dep472.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
Y. Xiao, X. Gao, S. Maragh, W. G. Telford, and A. Tona
Cell Lines as Candidate Reference Materials for Quality Control of ERBB2 Amplification and Expression Assays in Breast Cancer
Clin. Chem., July 1, 2009; 55(7): 1307 - 1315.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. R. Sompuram, G. Bastas, K. Vani, and S. A. Bogen
Accurate identification of paraprotein antigen targets by epitope reconstruction
Blood, January 1, 2008; 111(1): 302 - 308.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2002

 
Purchase HCS Short Course Manual on HCS site