Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Strik, M. C.
Right arrow Articles by Kummer, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Strik, M. C.
Right arrow Articles by Kummer, J. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 50, 1443-1454, November 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

Distribution of the Human Intracellular Serpin Protease Inhibitor 8 in Human Tissues

Merel C. Strika, Bellinda A. Bladergroena, Dorine Woutersa, Walter Kisielc, Jan Hendrik Hooijberga, Angelique R. Verlaanb, Peter L. Hordijke, Pascal Schneiderd, C. Erik Hacka,f, and J. Alain Kummerb,d
a VU University Medical Center, Departments of Clinical Chemistry, Amsterdam, The Netherlands
b Pathology, Amsterdam, The Netherlands
c Department of Pathology, University of New Mexico School of Medicine, Albuquerque, New Mexico
d Institute of Biochemistry, BIL Biomedical Research Center, University of Lausanne, Epalinges, Switzerland
e CLB, Departments of Experimental Immunohematology, Sanquin Blood Supply Foundation, Amsterdam, The Netherlands
f Immunopathology, Sanquin Blood Supply Foundation, Amsterdam, The Netherlands

Correspondence to: Merel C. Strik, Dept. of Clinical Chemistry, VU University Medical Center, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands. E-mail: m.strik@vumc.nl

Ovalbumin-like serine protease inhibitors are mainly localized intracellularly and their in vivo functions are largely unknown. To elucidate their physiological role(s), we studied the expression of one of these inhibitors, protease inhibitor 8 (PI-8), in normal human tissues by immunohistochemistry using a PI-8-specific monoclonal antibody. PI-8 was strongly expressed in the nuclei of squamous epithelium of mouth, pharynx, esophagus, and epidermis, and by the epithelial layer of skin appendages, particularly by more differentiated epithelial cells. PI-8 was also expressed by monocytes and by neuroendocrine cells in the pituitary gland, pancreas, and digestive tract. Monocytes showed nuclear and cytoplasmic localization of PI-8, whereas neuroendocrine cells showed only cytoplasmic staining. In vitro nuclear localization of PI-8 was confirmed by confocal analysis using serpin-transfected HeLa cells. Furthermore, mutation of the P1 residue did not affect the subcellular distribution pattern of PI-8, indicating that its nuclear localization is independent of the interaction with its target protease. We conclude that PI-8 has a unique distribution pattern in human tissues compared to the distribution patterns of other intracellular serpins. Additional studies must be performed to elucidate its physiological role.

(J Histochem Cytochem 50:1443–1453, 2002)

Key Words: serpin, human, nucleus, PI-8, immunohistochemistry


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
M. Bots and J. P. Medema
Serpins in T cell immunity
J. Leukoc. Biol., November 1, 2008; 84(5): 1238 - 1247.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. W. Stout-Delgado, Y. Getachew, B. C. Miller, and D. L. Thiele
Intrahepatic Lymphocyte Expression of Dipeptidyl Peptidase I-Processed Granzyme B and Perforin Induces Hepatocyte Expression of Serine Proteinase Inhibitor 6 (Serpinb9/SPI-6)
J. Immunol., November 15, 2007; 179(10): 6561 - 6567.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. C. M. Strik, A. Wolbink, D. Wouters, B. A. Bladergroen, A. R. Verlaan, I. S. van Houdt, S. Hijlkema, C. E. Hack, and J. A. Kummer
Intracellular serpin SERPINB6 (PI6) is abundantly expressed by human mast cells and forms complexes with {beta}-tryptase monomers
Blood, April 1, 2004; 103(7): 2710 - 2717.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2002