Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Verburg, M.
Right arrow Articles by Dekker, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Verburg, M.
Right arrow Articles by Dekker, J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 50, 1525-1536, November 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

Specific Responses in Rat Small Intestinal Epithelial mRNA Expression and Protein Levels During Chemotherapeutic Damage and Regeneration

Melissa Verburga, Ingrid B. Renesa, Danielle J.P.M. Van Nispena, Sacha Ferdinandussea, Marieke Jorritsmaa, Hans A. Büllerb, Alexandra W.C. Einerhanda, and Jan Dekkera
a Pediatric Gastroenterology and Nutrition, Department of Pediatrics, Erasmus University, Rotterdam, The Netherlands
b Sophia Children's Hospital, Rotterdam, The Netherlands

Correspondence to: Jan Dekker, Laboratory of Pediatrics, Room Ee1571A, Erasmus University, Dr Molewaterplein 50, 3015GE Rotterdam, The Netherlands. E-mail: dekker@kgk.fgg.eur.nl

The rapidly dividing small intestinal epithelium is very sensitive to the cytostatic drug methotrexate. We investigated the regulation of epithelial gene expression in rat jejunum during methotrexate-induced damage and regeneration. Ten differentiation markers were localized on tissue sections and quantified at mRNA and protein levels relative to control levels. We analyzed correlations in temporal expression patterns between markers. mRNA expression of enterocyte and goblet cell markers decreased significantly during damage for a specific period. Of these, sucrase-isomaltase (-62%) and CPS (-82%) were correlated. Correlations were also found between lactase (-76%) and SGLT1 (-77%) and between I-FABP (-52%) and L-FABP (-45%). Decreases in GLUT5 (-53%), MUC2 (-43%), and TFF3 (-54%) mRNAs occurred independently of any of the other markers. In contrast, lysozyme mRNA present in Paneth cells increased (+76%). At the protein level, qualitative and quantitative changes were in agreement with mRNA expression, except for Muc2 (+115%) and TFF3 (+81%), which increased significantly during damage, following independent patterns. During regeneration, expression of each marker returned to control levels. The enhanced expression of cytoprotective molecules (Muc2, TFF3, lysozyme) during damage represents maintenance of goblet cell and Paneth cell functions, most likely to protect the epithelium. Decreased expression of enterocyte-specific markers represents decreased enterocyte function, of which fatty acid transporters were least affected.

(J Histochem Cytochem 50:1525–1536, 2002)

Key Words: methotrexate, intestinal function, malabsorption, diarrhea


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Ann OncolHome page
J. P. M. Derikx, N. M. A. Blijlevens, J. P. Donnelly, H. Fujii, T. Kanda, A. A. van Bijnen, E. Heineman, and W. A. Buurman
Loss of enterocyte mass is accompanied by diminished turnover of enterocytes after myeloablative therapy in haematopoietic stem-cell transplant recipients
Ann. Onc., August 25, 2008; (2008) mdn579v1.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. W. A. Rossen, J. Bouma, R. H. C. Raatgeep, H. A. Buller, and A. W. C. Einerhand
Inhibition of Cyclooxygenase Activity Reduces Rotavirus Infection at a Postbinding Step
J. Virol., September 15, 2004; 78(18): 9721 - 9730.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2002