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Journal of Histochemistry and Cytochemistry, Vol. 50, 1647-1657, December 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

The mt1 Melatonin Receptor and RORß Receptor Are Co-localized in Specific TSH-immunoreactive Cells in the Pars Tuberalis of the Rat Pituitary

Paul Klosena, Christele Bienvenua, Olivier Demarteaua, Hugues Dardentea, Hilda Guerreroa, Paul Péveta, and Mireille Masson–Péveta
a Neurobiologie des Rythmes, CNRS-UMR 7518, IFR 37, Université Louis Pasteur, Strasbourg, France

Correspondence to: Paul Klosen, Neurobiologie des Rythmes, CNRS-UMR 7518, 12, rue de l'Université, 67 000 Strasbourg, France. E-mail: klosen@neurochem.u-strasbg.fr

The pars tuberalis (PT) of the pituitary represents an important target site for the time-pacing pineal hormone melatonin because it expresses a large number of mt1 receptors. Functional studies suggest that the PT mediates the seasonal effects of melatonin on prolactin (PRL) secretion. The aim of this study was the characterization of the phenotype of melatonin-responsive cells. Furthermore, we determined whether RORß, a retinoid orphan receptor present in the PT, was co-expressed in the same cells. We combined nonradioactive in situ hybridization (ISH) with hapten-labeled riboprobes for detection of the receptors and immunocytochemistry (ICC) for detection of {alpha}GSU ({alpha}-glycoprotein subunit), ßTSH, ßFSH, ßLH, GH, PRL, and ACTH. Expression of mt1 mRNA was found in small round cells, co-localized with {alpha}GSU and ßTSH. However, not all ßTSH-containing cells expressed mt1 mRNA. The distribution of mt1- and RORß-positive cells appeared to overlap, although more cells were labeled for RORß than for mt1. Gonadotrophs, as well as other pars distalis cell types, were never labeled for mt1 melatonin receptor. Therefore, this study identifies the "specific" cells of the PT as the mt1 melatonin receptor-expressing cells.

(J Histochem Cytochem 50:1647–1657, 2002)

Key Words: mt1 melatonin receptor, RORß receptor, pituitary, pars tuberalis, in situ hybridization, immunocytochemistry


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