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Journal of Histochemistry and Cytochemistry, Vol. 50, 1693-1696, December 2002, Copyright © 2002, The Histochemical Society, Inc.

BRIEF REPORT

Effect of Fixation Period on HER2/neu Gene Amplification Detected by Fluorescence In Situ Hybridization in Invasive Breast Carcinoma

Sathiyamoorthy Selvarajana, Boon-Huat Baya, Andrew Choob, Khoon-Leong Chuahb, Christina Rudduck Sivaswarenb, Sim-Leng Tienb, Chow-Yin Wongc, and Puay-Hoon Tanb
a Department of Anatomy, National University of Singapore, Singapore General Hospital, Singapore
b Departments of Pathology, Singapore General Hospital, Singapore
c General Surgery, Singapore General Hospital, Singapore

Correspondence to: Puay-Hoon Tan, Dept. of Pathology, Singapore General Hospital, Outram Road, Singapore 169608. E-mail: gpttph@sgh.com.sg

This study investigated if formalin fixation duration affects HER2/neu gene amplification detection by fluorescence in situ hybridization (FISH) in breast cancer. Tumor tissues from 35 cases were divided into three groups and subjected to two formalin fixation protocols per group (12 hr, 27 hr in the first; 2 hr, 17.5 hr in the second; 28.5 hr, 541 hr in the third) before FISH analysis. There was no significant difference in FISH signal detection between the two different fixation protocols in the first two groups. In the third, no signal was detected in 4/6 cases fixed for an extended duration.

(J Histochem Cytochem 50:1693–1696, 2002)

Key Words: FISH, HER2/neu, fixation, breast cancer, immunohistochemistry


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