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Journal of Histochemistry and Cytochemistry, Vol. 50, 1697-1698, December 2002, Copyright © 2002, The Histochemical Society, Inc.

BRIEF REPORT

Suspension (S)-FISH, a New Technique for Interphase Nuclei

Ulf Steinhaeusera, Heike Starkea, Angela Nietzela, Joerg Lindenaub, Peter Ullmannb, Uwe Claussena, and Thomas Liehra
a Institute of Human Genetics, Jena, Germany
b Carl Zeiss Jena GmbH, Jena, Germany

Correspondence to: Thomas Liehr, Institut für Humangenetik, Postfach, D-07740 Jena, Germany. E-mail: i8lith@mti-n.mti.uni-jena.de

We describe a versatile method for performing fluorescence in situ hybridization (FISH) in suspension instead of on a slide as usually done. This so-called suspension-FISH (S-FISH) opens new possibilities for the analysis of shape and functions of the human interphase nucleus. The procedure is described and the first results using this approach are presented. (J Histochem Cytochem 50:1697–1698, 2002)

Key Words: FISH, suspension FISH (S-FISH), interphase, nuclear architecture


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