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Journal of Histochemistry and Cytochemistry, Vol. 50, 171-184, February 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

Myosin Heavy Chain Composition of Muscle Spindles in Human Biceps Brachii

Jing-Xia Liua,c, Per-Olof Erikssonb,c, Lars-Eric Thornella,c, and Fatima Pedrosa–Domellöfa,c
a Departments of Integrative Medical Biology, Section of Anatomy, Umeå, Sweden
b Clinical Oral Physiology, Umeå, Sweden
c Umeå University, and Department of Musculoskeletal Research, National Institute for Working Life, Umeå, Sweden

Correspondence to: Fatima Pedrosa–Domellöf, Dept. of Integrative Medical Biology, Section of Anatomy, Umeå University, SE-901 87 Umeå, Sweden. E-mail: Fatima.Pedrosa-Domellof@anatomy.umu.se

Data on the myosin heavy chain (MyHC) composition of human muscle spindles are scarce in spite of the well-known correlation between MyHC composition and functional properties of skeletal muscle fibers. The MyHC composition of intrafusal fibers from 36 spindles of human biceps brachii muscle was studied in detail by immunocytochemistry with a large battery of antibodies. The MyHC content of isolated muscle spindles was assessed with SDS-PAGE and immunoblots. Four major MyHC isoforms (MyHCI, IIa, embryonic, and intrafusal) were detected with SDS-PAGE. Immunocytochemistry revealed very complex staining patterns for each intrafusal fiber type. The bag1 fibers contained slow tonic MyHC along their entire fiber length and MyHCI, {alpha}-cardiac, embryonic, and fetal isoforms along a variable part of their length. The bag2 fibers contained MyHC slow tonic, I, {alpha}-cardiac, embryonic, and fetal isoforms with regional variations. Chain fibers contained MyHCIIa, embryonic, and fetal isoforms throughout the fiber, and MyHCIIx at least in the juxtaequatorial region. Virtually each muscle spindle had a different allotment of numbers of bag1, bag2 and chain fibers. Taken together, the complexity in intrafusal fiber content and MyHC composition observed indicate that each muscle spindle in the human biceps has a unique identity. (J Histochem Cytochem 50:171–183, 2002)

Key Words: human biceps brachii, muscle spindles, intrafusal fiber, bag1 fiber, bag2 fiber, chain fiber, MyHC


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