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Journal of Histochemistry and Cytochemistry, Vol. 50, 223-234, February 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

Fluid Dynamics of the Excretory Flow of Zymogenic and Mucin Contents in Rat Gastric Gland Processed by High-pressure Freezing/Freeze Substitution

Akira Sawaguchia, Kazuhiko Ishiharab, Jun-ichi Kawanoa, Tsutomu Oinumaa, Kyoko Hottac, and Tatsuo Suganumaa
a Department of Anatomy, Miyazaki Medical College, Miyazaki, Japan
b Department of Biochemistry, Kitasato University School of Allied Health Sciences, Kanagawa, Japan
c Department of Biochemistry, Kitasato University School of Medicine, Kanagawa, Japan

Correspondence to: Tatsuo Suganuma, Dept. of Anatomy, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki 889-1692, Japan. E-mail: suganumat@post1.miyazaki-med.ac.jp

The high-pressure freezing/freeze substitution technique followed by Lowicryl K4M embedding provided an excellent ultrastructure and retention of antigenicity of rat gastric glands as well as the intraluminal fluid contents. By taking this advantage, we histochemically investigated the excretory flow of the zymogenic and mucin contents in rat gastric glandular lumen at the ultrastructural level. The combination of KMnO4-UA/Pb staining for zymogenic contents and Griffonia simplicifolia agglutinin-II (GSA-II) labeling for mucous neck cell (MNC) mucin distinguished the exocytosed zymogenic contents from the MNC mucin in the glandular lumen. Interestingly, at the base and neck regions, the zymogenic contents showed a droplet-like appearance, forming a distinct interface with the MNC mucin. At the pit region, the GSA-II labeling demonstrated restricted paths, designated as MNC mucous channels, which flowed into the surface mucous gel layer. It should be noted that the interface between exocytosed zymogenic contents and MNC mucin disappeared, and that the zymogenic contents merged into the MNC mucous channels. At the top pit region, the surface mucous gel layer showed laminated arrays of three types of gastric mucins. On the basis of these ultrastructural findings, we propose a model of the excretory flow in rat gastric gland. (J Histochem Cytochem 50:223–234, 2002)

Key Words: high-pressure freezing, gastric gland, fluid dynamics, excretory flow, intraluminal channel, zymogenic contents, mucin contents, immunogold labeling, permanganate oxidation


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