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Journal of Histochemistry and Cytochemistry, Vol. 50, 265-274, February 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

Localization of Two Enzymes of the Tetrahydrobiopterin Biosynthetic Pathway in Embryonic Chick Retina

Vered Holdengrebera, Cornelia Kriegera, Markus Gütlichb, Nikolas Schramekb, Michal Vechoropoulosa, Markus Fischerb, Adelbert Bacherb, and Yehuda Ben-Shaula
a Tel-Aviv University, Department of Cell Research and Immunology, Tel Aviv, Israel
b Technical University of Munich, Institute of Organic Chemistry and Biochemistry, Garching, Germany

Correspondence to: Yehuda Ben-Shaul, Tel-Aviv University, Dept. of Cell Research and Immunology, Tel Aviv 69978, Israel. E-mail: yehudab@post.tau.ac.il

Tetrahydrobiopterin (BH4) is an essential co-factor for the biosynthesis of catecholamine-type neurotransmitters and of nitric oxide (NO). The expression of the enzymes catalyzing the first two steps of the BH4 biosynthetic pathway was studied in the developing chicken retina by in situ hybridization and immunocytochemistry. GTP-cyclohydrolase-I (GTP-CH-I) and 6-pyruvoyl-tetrahydropterin synthase (PTPS) were already expressed in the undifferentiated and proliferating retina of E7. At stage E11 both enzymes were expressed in photoreceptors, amacrine cells, displaced amacrine cells, and ganglion cells, and in the plexiform layers in which synaptic connections take place. At stage E18 the labeling was comparable to E11 but appeared to be more concentrated in photoreceptors and ganglion cells. (J Histochem Cytochem 50:265–274, 2002)

Key Words: BH4, chick retina, development, PTPS, GTP-cyclohydrolase I, in situ hybridization, immunolabeling, tyrosine hydroxylase, nitric oxide synthase


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