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Journal of Histochemistry and Cytochemistry, Vol. 50, 289-303, March 2002, Copyright © 2002, The Histochemical Society, Inc.

REVIEW

Fluorescent Histochemical Techniques for Analysis of Intracellular Signaling

Morten P. Oksvolda, Ellen Skarpena, Jannicke Widerberga, and Henrik S. Huitfeldta
a Center for Cellular Stress Responses, Institute of Pathology, University of Oslo, Oslo, Norway

Correspondence to: Morten P. Oksvold, Center for Cellular Stress Responses, Inst. of Pathology, U. of Oslo, Rikshospitalet, N-0027, Oslo, Norway. E-mail: m.p.oksvold@labmed.uio.no

Intracellular signaling relies on the orchestrated cooperation of signaling proteins and modules, their intracellular localization, and membrane trafficking. Recently, a repertoire of fluorescence-based techniques, which significantly increases our potential for detailed studies of the involved mechanisms, has been introduced. Microscopic techniques with increased resolution have been combined with improved techniques for detection of signaling proteins. Transfections of fluorescently tagged proteins have allowed in vivo microscopy of their trafficking and interactions with other proteins and intracellular structures. We present an overview of general signaling principles and a description of techniques based on fluorescent microscopy suited for studies of signaling mechanisms.

(J Histochem Cytochem 50:289–303, 2002)

Key Words: fluorescence microscopy, extended resolution, protein interactions, intracellular signaling


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