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Journal of Histochemistry and Cytochemistry, Vol. 50, 395-403, March 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

IGF-1 Induces Foam Cell Formation in Rat Glomerular Mesangial Cells

Anne K. Berfielda and Christine K. Abrassa
a Veteran Affairs Puget Sound Health Care System, Seattle, Washington

Correspondence to: Christine K. Abrass, Veteran Affairs Puget Sound Health Care System, 1660 S. Columbian Way, Seattle, WA 98108. E-mail: cabrass@u.washington.edu

When rat glomerular mesangial cells (MCs) are cultured with IGF-1 they accumulate intracellular lipid and take on foam cell morphology. These changes were characterized by electron microscopy and Nile red staining. To define the mechanism responsible for IGF-1-mediated lipid uptake, MCs were evaluated for endocytosis, scavenger receptor activity, and receptor-mediated uptake by the LDL receptor. Lipid accumulation was markedly increased when MCs were cultured with IGF. The primary route of uptake was through enhanced endocytosis. Lipid-laden MCs have decreased phagocytic capacity and disrupted cytoskeletons. These data show that IGF-1 induces MC to take on a foam cell morphology and that lipid-laden MCs have impaired phagocytic function. (J Histochem Cytochem 50:395–403, 2002)

Key Words: mesangial cell, IGF-1, atherosclerosis, lipids, glomerulosclerosis, phagocytosis


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 Am. J. Physiol. Renal Physiol.Home page
A. K. Berfield, A. Chait, J. F. Oram, R. A. Zager, A. C. Johnson, and C. K. Abrass
IGF-1 induces rat glomerular mesangial cells to accumulate triglyceride
Am J Physiol Renal Physiol, January 1, 2006; 290(1): F138 - F147.
[Abstract] [Full Text] [PDF]



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