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Journal of Histochemistry and Cytochemistry, Vol. 50, 473-482, April 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

Expression of Invariant Chain (CD 74) and Major Histocompatibility Complex (MHC) Class II Antigens in the Human Fetus

Sunil Badvea,b, Charuhas Deshpandeb, Zhongxue Huaa,c, and Lennart Lögdberga,d
a Department of Pathology, Albert Einstein College of Medicine, Bronx, New York
b Division of Surgical Pathology, Northwestern University, Chicago, Illinois
c Office of the State Medical Examiner, Newark, New Jersey
d Transfusion Medicine Program, Department of Pathology, Emory University, Atlanta, Georgia

Correspondence to: Sunil Badve, Div. of Surgical Pathology, Feinberg Pavilion 7-325, Northwestern Memorial Hospital, 251 East Huron, Chicago, IL 60611. E-mail: s-badve@northwestern.edu

During the initiation of an immune response, antigen-presenting cells employ MHC class II antigens as key molecules to present small peptides to CD4-positive lymphocytes. The invariant chain (Ii; CD74) plays a critical role in this process by influencing the expression and peptide loading of the MHC class II molecules. Therefore, coordinate expression of these molecules is believed to play an important role in antigen presentation. This study explores the expression of these molecules in fetal tissues. Formalin-fixed, paraffin-embedded multi-organ tissue blocks from aborted fetuses (age range 7–22 weeks) were immunostained for Ii/CD74 and MHC class II antigens using commercially available monoclonal antibodies for Ii/CD74 (LN2) and MHC class II antigens (LN3), respectively. Coordinate staining for Ii/CD74 and MHC class II antigens was seen in the skin, proximal renal tubules, tips of small intestinal mucosa, and cells of the reticuloendothelial system, including the spleen and thymus. Expression of Ii/CD74, but not of MHC class II antigens, was seen in pulmonary alveolar epithelium in all cases and in testicular Leydig cells (11 of 11 testes examined). The distribution and intensity of staining did not change significantly with age. In conclusion, this study describes distribution of Ii/CD74 and MHC class II antigens in human fetal tissues. Coordinate expression of Ii/CD74 and MHC class II antigens was identified in most fetal tissues, but there were also notable exceptions. In all cases this took the form of expression of Ii/CD74 in the absence of MHC class II expression. Discordance was particularly striking in pulmonary alveolar epithelium and testicular Leydig cells. This suggests that the Ii/CD74 molecule has functional roles in addition to its role in antigen presentation.

(J Histochem Cytochem 50:473–482, 2002)

Key Words: fetal tissues, MHC class II, invariant chain, CD74, LN2, LN3, discordance


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