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Journal of Histochemistry and Cytochemistry, Vol. 50, 709-718, May 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

Ganglion Cells and Topographically Related Nerves in the Vallate Papilla/von Ebner Gland Complex

Andrea Sbarbatia, Flavia Merigoa, Paolo Bernardia, Caterina Crescimannoa, Donatella Benatia, and Francesco Osculatia
a Department of Morphological and Biomedical Sciences, Section of Anatomy and Histology, University of Verona, Verona, Italy

Correspondence to: Andrea Sbarbati, Dept. of Morphological and Biomedical Sciences, Section of Anatomy and Histology, University of Verona, Medical Faculty, Strada Le Grazie 8, 37134 Verona, Italy. E-mail: SBARBATI@borgoroma.univr.it

Ganglion cells and topographically related nerves in the vallate papilla/von Ebner gland complex were investigated in rat tongue by cytochemical, immunocytochemical, and ultrastructural methods to evaluate the possible presence of different neuronal subpopulations. Immunostaining for neurofilaments and protein gene product 9.5 revealed ganglionic cell bodies and nerve fibers. A large part of the neurons were positive at immunostaining for neuronal nitric oxide synthase (NOS), vesicular acetylcholine transporter (VAChT), or vasoactive intestinal peptide (VIP). A small subset of nerve fibers revealed immunoreactivity for cholecystokinin. Axons traveling under the lingual epithelium were evidenced by their content of calcitonin gene-related peptide (CGRP) or substance P (SP). Cell bodies positive for SP or CGRP were not detected. Using methods of co-localization, three different neuronal classes were detected. The main population was composed of AChE/NADPH-diaphorase (NADPHd)-positive cells. Small groups of acetylcholine esterase (AChE)-positive/NADPHd-negative cells were visible. Isolated neurons were AChE-negative/NADPHd-positive. The results of co-localization experiments for VAChT/NOS were consistent with those obtained by cytochemical co-localization of AChE and NADPHd. Experiments of co-localization for peptidergic and nitrergic structures revealed CGRP- and SP-immunoreactive fibers in the vallate papilla/von Ebner gland ganglion. In conclusion, the results demonstrated in the VP/VEG complex peptidergic, cholinergic, and nitrergic neurons. The presence of different neuronal subclasses suggests that a certain degree of functional specialization may exist. (J Histochem Cytochem 50:709–718, 2002)

Key Words: neurotransmitter, NADPH-diaphorase, acetylcholine esterase, immunohistochemistry, taste, tongue


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[Abstract] [Full Text] [PDF]



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