Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Janssen, S. W.J.
Right arrow Articles by Bindels, R. J.M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Janssen, S. W.J.
Right arrow Articles by Bindels, R. J.M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 50, 789-798, June 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

Expression of the Novel Epithelial Ca2+ Channel ECaC1 in Rat Pancreatic Islets

Susan W.J. Janssena,b, Joost G.J. Hoenderopc, Ad R.M.M. Hermusb, Fred C.G.J. Sweepd, Gerard J.M. Martensa, and René J.M. Bindelsc
a Department of Animal Physiology, Faculty of Science, University of Nijmegen, Nijmegen, The Netherlands, University Medical Centre Nijmegen, Nijmegen, The Netherlands
b Departments of Endocrinology, University Medical Centre Nijmegen, Nijmegen, The Netherlands
c Cell Physiology, University Medical Centre Nijmegen, Nijmegen, The Netherlands
d Chemical Endocrinology, University Medical Centre Nijmegen, Nijmegen, The Netherlands

Correspondence to: René J.M. Bindels, 160 Cell Physiology, University Medical Centre Nijmegen, PO Box 9101, NL-6500 HB Nijmegen, The Netherlands. E-mail: reneb@sci.kun.nl

The epithelial Ca2+ channel, ECaC1, is primarily expressed in the apical membrane of vitamin D-responsive tissues. This study characterizes for the first time the presence of this novel channel in pancreatic tissue by reverse transcriptase-polymerase chain reaction and immunohistochemistry. In addition, the expression of ECaC1 was investigated in an animal model for Type 2 diabetes mellitus, the Zucker diabetic fatty (ZDF) rat. Identical staining patterns for ECaC1 and insulin were observed, whereas no co-localization of ECaC1 with glucagon was found. ECaC1, insulin, and prohormone convertase 1 (a neuroendocrine endoprotease expressed in secretory granules) showed a similar punctate staining. ECaC1 co-localized with the Ca2+ binding protein calbindin-D28K in the ß-cells. Furthermore, in contrast to wild-type rats, in ZDF rats aging led to a progressive decrease in both insulin and ECaC1 staining. Plasma 1,25-dihydroxyvitamin D3 levels were similar in both control and ZDF rats and decreased with aging. Taken together, our findings indicate that this novel Ca2+ channel may play a role in the regulation of endocrine Ca2+ homeostasis. (J Histochem Cytochem 50:789–798, 2002)

Key Words: ß-cell, calbindin-D28K, 1,25-dihydroxyvitamin D3, NIDDM, ZDF rat


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
B. Nilius, G. Owsianik, T. Voets, and J. A. Peters
Transient Receptor Potential Cation Channels in Disease
Physiol Rev, January 1, 2007; 87(1): 165 - 217.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Döhr, A. Klingenhoff, H. Maier, M. H. de Angelis, T. Werner, and R. Schneider
Linking disease-associated genes to regulatory networks via promoter organization
Nucleic Acids Res., February 8, 2005; 33(3): 864 - 872.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
J. G. J. Hoenderop, B. Nilius, and R. J. M. Bindels
Calcium Absorption Across Epithelia
Physiol Rev, January 1, 2005; 85(1): 373 - 422.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2002