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Journal of Histochemistry and Cytochemistry, Vol. 50, 903-910, July 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

Immunocytochemical Localization of Prohormone Convertases PC1 and PC2 in the Mouse Thyroid Gland and Respiratory Tract

Shingo Kurabuchia and Shigeyasu Tanakab
a Department of Histology, School of Dentistry, The Nippon Dental University, Tokyo, Japan
b Department of Biology, Faculty of Science, Shizuoka University, Shizuoka, Japan

Correspondence to: Shigeyasu Tanaka, Dept. of Biology, Faculty of Science, Shizuoka University, Ohya 836, Shizuoka 422-8529, Japan. E-mail: sbstana@ipc.shizuoka.ac.jp

We examined immunocytochemical localization of the prohormone convertases, PC1 and PC2, in the thyroid gland and respiratory tract of the adult mouse using the indirect enzyme- and immunogold-labeled antibody methods for light and electron microscopy, respectively. In the thyroid gland, PC1- and/or PC2-immunoreactive cells were cuboidal, scattered in the follicular epithelium and in the interfollicular spaces. When serial sections were immunostained with anti-calcitonin, anti-PC1, anti-calcitonin-gene-related-peptide (CGRP), and anti-PC2 sera, respectively, localization of both PC1 and PC2 was restricted to the calcitonin/CGRP-producing parafollicular cells. In the respiratory tract, only PC1 immunoreactivity was observed in the basal granulated neuroendocrine cells, which were scattered in the tracheal epithelium. Consecutive sections immunostained with anti-PC1 and anti-CGRP sera showed that a subpopulation of these PC1-immunoreactive cells contained CGRP. Double immunogold electron microscopy of the thyroid parafollicular cells revealed that calcitonin- and/or CGRP-immunopositive secretory granules were also labeled with both PC1 and PC2. These findings suggest that procalcitonin is proteolytically cleaved by PC2 alone or by PC2 together with PC1, and that the proCGRP is cleaved by PC1.

(J Histochem Cytochem 50:903–909, 2002)

Key Words: PC1, PC2, parafollicular cell (C-cell), thyroid gland, basal granulated cell, respiratory tract, immunocytochemistry, mouse


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