Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Briane, D.
Right arrow Articles by Taillandier, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Briane, D.
Right arrow Articles by Taillandier, E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 50, 983-992, July 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

Cellular Pathway of Plasmids Vectorized by Cholesterol-based Cationic Liposomes

Dominique Brianea,c, Denis Lesagea, An Caoa, Robert Couderta,b, Nicole Lievred, Jean Loup Salzmannd, and Eliane Taillandiera
a Laboratoire de Chimie Structurale et Spectroscopie Biomoléculaire, CNRS UPRES-A 7031, UFR SMBH (Santé, Médecine et Biologie Humaine), Université Paris XIII, Bobigny, France
b Laboratoire de Physicochimie des Interfaces et des Milieux Réactionnels, PIMIR, EA 2098, Faculté des Sciences, Tours, France
c Laboratoire d'Oncologie Cellulaire et Moléculaire, EA 2360, Université Paris XIII, UFR SMBH, Bobigny, France
d Laboratoire Biotherapies: Benefices et Risques, EA 3410, UFR SMBH, Université Paris XIII, Bobigny, France

Correspondence to: An Cao, Laboratoire de Chimie Structurale et Spectroscopie Biomoléculaire, CNRS UMR 7033, UFR de Médecine, Université Paris XIII, 74 rue Marcel Cachin, F93017 Bobigny Cedex, France. E-mail: cao@smbh.univ-paris13.fr

We investigated by transmission electron microscopy the cellular route in tumor MCF7 cells of DNA labeled with digoxigenin, carried by cationic liposomes (Lip+) prepared from TMAEC-Chol [3ß(N-(N',N',N'-trimethylaminoethane)-carbamoyl)cholesterol iodide] and TEAPC-Chol [3ß(N-(N',N',N'-triethylaminopropane)-carbamoyl)cholesterol iodide], two cholesterol-based cationic lipids containing a quaternary ammonium. In a previous work we showed the pathway of cationic lipid/plasmid complexes from the beginning of endocytosis until their entry into the perinuclear area. Beyond this limit, unlabeled exogenous plasmids cannot be distinguished with nuclear DNA. This work dealt with the cellular fate of cationic liposome-vectorized plasmids labeled with digoxigenin using an immunogold procedure. Early after the beginning of transfection (30 min, 1 hr, 5 hr), gold particles were observed only in the cytoplasm and in endosome-like vesicles, whereas after 24 hr gold particles were densely present in the nucleus. These results demonstrate the nuclear localization of plasmids vectorized by the cationic liposomes used. The results are discussed in comparison with transfection efficiency measurements.

(J Histochem Cytochem 50:983–991, 2002)

Key Words: cationic lipids, plasmids, MCF7 cells, digoxigenin, immunogold electron, microscopy, gene transfer


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Immunol.Home page
K. Zaks, M. Jordan, A. Guth, K. Sellins, R. Kedl, A. Izzo, C. Bosio, and S. Dow
Efficient Immunization and Cross-Priming by Vaccine Adjuvants Containing TLR3 or TLR9 Agonists Complexed to Cationic Liposomes.
J. Immunol., June 15, 2006; 176(12): 7335 - 7345.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2002

 
Purchase HCS Short Course Manual on HCS site